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- W1978373965 abstract "Free and N-acetylated hexosamines can be determined spectrophotometrically by a three-stage assay: chlorinating the amide or amine with NaOCl; reducing unreacted NaOCl with NaNO2; and reacting chloroamide (-amine) with amylose-KI reagent to produce the blue amylosetriiodide complex, measured at 615 nm. Distinctive behavior of the common 2-amino-2-deoxy-d-hexoses and their 2-acetyl derivatives allows three types of measurements to be made: (a) identification and differentiation by characteristic behavior (assay individually in primary cacodylate buffers over the pH range 6.0–9.5, then adjust pH to 5.5 with secondary phthalate buffer and remeasure the color); (b) assay individually in a single buffer at the optimum pH; and (c) assay differently admixed 2-amino-2-deoxy-d-hexose · HCl and 2-acctamindo-2-deoxy-d-hexose (assay in primary buffer at pH 9.0–9.5 gives measure of only HexNAc (no reaction with ManNAc), then adjust pH to 5.5 with secondary buffer to measure additional color that results only from HexN · HCl)." @default.
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- W1978373965 date "1971-08-01" @default.
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- W1978373965 title "Reaction of sodium hypochlorite with amines and amides: A new method for quantitating amino sugars in monomeric form" @default.
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- W1978373965 doi "https://doi.org/10.1016/0003-2697(71)90056-x" @default.
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