Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978377273> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W1978377273 endingPage "161" @default.
- W1978377273 startingPage "153" @default.
- W1978377273 abstract "A time-resolved fluoroimmunoassay (TR-FIA) for human salivary cortisol was adapted for the measurement of cortisol in unextracted bovine blood plasma and serum. It has been demonstrated that the binding of cortisol binding plasma proteins (CBPP) to the cortisol–biotin primary probe cannot be eliminated by means of cortisol releasing agents. Complete inactivation of CBPP was achieved by heating water diluted samples for 30 min at 80°C. The high non-specific binding (NSB) of the streptavidin–europium secondary probe, encountered during preliminary experiments, was shown to be caused by an interaction with bovine serum albumin (BSA) and could be reduced partly by the addition of heparin. It was also shown that the ability to bind streptavidin–europium nonspecifically is not a general property of proteins since bovine γ-globulin and gelatin lack this behaviour. The advantage of a highly reduced NSB, resulting from the use of a BSA free assay buffer, is not limited to this particular assay but is also beneficial for other procedures based on specific measurement of streptavidin–europium fluorescence. The detection limit for a 20 μl sample was 0.5 ng/ml. The intra-assay coefficients of variation for control samples with cortisol concentrations of 71.1, 39.2 and 10.3 ng/ml were 8.2, 7.9 and 11.3% (n=16). The corresponding inter-assay coefficients of variation were 7.3, 9.0 and 11.2% (n=73). Correlation with a commercially available radioimmunoassay, preceded by diethylether extraction of the sample, was 0.97 (n=88)." @default.
- W1978377273 created "2016-06-24" @default.
- W1978377273 creator A5018294945 @default.
- W1978377273 creator A5027672153 @default.
- W1978377273 creator A5038389535 @default.
- W1978377273 creator A5065698798 @default.
- W1978377273 date "1998-10-01" @default.
- W1978377273 modified "2023-10-16" @default.
- W1978377273 title "A time-resolved fluoroimmunoassay for cortisol in unextracted bovine plasma or serum with optimized procedures to eliminate steroid binding protein interference and to minimize non-specific streptavidin–europium binding" @default.
- W1978377273 cites W1938467486 @default.
- W1978377273 cites W2036643530 @default.
- W1978377273 cites W2048809331 @default.
- W1978377273 cites W2053993382 @default.
- W1978377273 cites W2073915689 @default.
- W1978377273 cites W2318130783 @default.
- W1978377273 cites W4255423078 @default.
- W1978377273 doi "https://doi.org/10.1016/s0960-0760(98)00083-1" @default.
- W1978377273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9877216" @default.
- W1978377273 hasPublicationYear "1998" @default.
- W1978377273 type Work @default.
- W1978377273 sameAs 1978377273 @default.
- W1978377273 citedByCount "36" @default.
- W1978377273 countsByYear W19783772732017 @default.
- W1978377273 countsByYear W19783772732020 @default.
- W1978377273 crossrefType "journal-article" @default.
- W1978377273 hasAuthorship W1978377273A5018294945 @default.
- W1978377273 hasAuthorship W1978377273A5027672153 @default.
- W1978377273 hasAuthorship W1978377273A5038389535 @default.
- W1978377273 hasAuthorship W1978377273A5065698798 @default.
- W1978377273 hasConcept C145148216 @default.
- W1978377273 hasConcept C159654299 @default.
- W1978377273 hasConcept C176685330 @default.
- W1978377273 hasConcept C178790620 @default.
- W1978377273 hasConcept C185592680 @default.
- W1978377273 hasConcept C203014093 @default.
- W1978377273 hasConcept C2776190903 @default.
- W1978377273 hasConcept C2778204606 @default.
- W1978377273 hasConcept C2780420688 @default.
- W1978377273 hasConcept C43617362 @default.
- W1978377273 hasConcept C502696234 @default.
- W1978377273 hasConcept C55493867 @default.
- W1978377273 hasConcept C86803240 @default.
- W1978377273 hasConcept C86807702 @default.
- W1978377273 hasConceptScore W1978377273C145148216 @default.
- W1978377273 hasConceptScore W1978377273C159654299 @default.
- W1978377273 hasConceptScore W1978377273C176685330 @default.
- W1978377273 hasConceptScore W1978377273C178790620 @default.
- W1978377273 hasConceptScore W1978377273C185592680 @default.
- W1978377273 hasConceptScore W1978377273C203014093 @default.
- W1978377273 hasConceptScore W1978377273C2776190903 @default.
- W1978377273 hasConceptScore W1978377273C2778204606 @default.
- W1978377273 hasConceptScore W1978377273C2780420688 @default.
- W1978377273 hasConceptScore W1978377273C43617362 @default.
- W1978377273 hasConceptScore W1978377273C502696234 @default.
- W1978377273 hasConceptScore W1978377273C55493867 @default.
- W1978377273 hasConceptScore W1978377273C86803240 @default.
- W1978377273 hasConceptScore W1978377273C86807702 @default.
- W1978377273 hasIssue "2" @default.
- W1978377273 hasLocation W19783772731 @default.
- W1978377273 hasLocation W19783772732 @default.
- W1978377273 hasOpenAccess W1978377273 @default.
- W1978377273 hasPrimaryLocation W19783772731 @default.
- W1978377273 hasRelatedWork W151847383 @default.
- W1978377273 hasRelatedWork W1965960192 @default.
- W1978377273 hasRelatedWork W1969688909 @default.
- W1978377273 hasRelatedWork W1981156161 @default.
- W1978377273 hasRelatedWork W1994420353 @default.
- W1978377273 hasRelatedWork W2019687444 @default.
- W1978377273 hasRelatedWork W2077671099 @default.
- W1978377273 hasRelatedWork W2085791271 @default.
- W1978377273 hasRelatedWork W2276411880 @default.
- W1978377273 hasRelatedWork W2383901644 @default.
- W1978377273 hasVolume "67" @default.
- W1978377273 isParatext "false" @default.
- W1978377273 isRetracted "false" @default.
- W1978377273 magId "1978377273" @default.
- W1978377273 workType "article" @default.