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• W1978391237 abstract "Commercial antisera to human serum were coupled to Sepharose 4B or Staphylococcus aureus bacteria and used as immunoadsorbents to separate the serum-derived proteins in several types of body fluids from the fluid-specific, non-serum derived proteins. Two-dimensional gel analysis of fluids after incubation with one or the other immunoadsorbent produced similar results. Cerebrospinal, prostatic and amniotic fluid each exhibited a characteristic array of probable fluid-specific protein families which were not detected in serum. Data from model experiments suggested that Sepharose 4B was a somewhat better matrix material than Staphylococcus aureus cells. Pretreatment of body fluids with anti-human serum immunoadsorbents should facilitate the detection of fluid-specific protein markers of disease in two-dimensional gels." @default.
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• W1978391237 date "1983-02-01" @default.
• W1978391237 modified "2023-10-18" @default.
• W1978391237 title "Detection of the major non-serum derived proteins of body fluids by immunodeletion and two-dimensional gel electrophoresis" @default.
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• W1978391237 doi "https://doi.org/10.1002/elps.1150040305" @default.
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