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• W1978391605 abstract "A multicenter study was conducted. A panel containing DNA from Histoplasma capsulatum, as well as negative and cross-reaction controls, was sent to five different laboratories, members of the MICOMOL network from CYTED Program. The objective was to assess the accuracy of different PCR protocols to detect H. capsulatum DNA. Seven different PCR protocols were tested. They were based on PCR techniques and used unicopy and multicopy targets. Most of these protocols (4/7) were able to detect the smallest amounts of fungal DNA (102 fg/μl). Overall sensitivity was 86% and specificity was 100%. The protocol based on a unicopy target (SCAR220) presented lower sensitivity (43%) but 100% specificity. The real-time protocols tested were highly reproducible, sensitive, and specific. Neither false positives nor cross-reactions were detected in any protocol. All laboratories were able to amplify H. capsulatum DNA, and real-time PCR seems to be a promising tool to efficiently detect this pathogen in clinical samples. Se realizó un estudio multicéntrico en el que participaron cinco laboratorios miembros de la red MICOMOL a partir del programa CYTED. Los participantes recibieron un panel que contenía muestras de ADN de Histoplasma capsulatum, al igual que paneles con muestras de control negativas y de reacciones cruzadas. el objetivo del presente estudio fue examinar la precisión de los diferentes protocolos de PCR para la detección de ADN de H. capsulatum. Se examinaron siete protocolos, todos ellos basados en técnicas de PCR, que empleaban como diana plásmidos unicopia o multicopia. la mayoría de estos protocolos (4/7) pudieron detectar las cantidades más pequeñas de ADN (102 fg/μl). La sensibilidad global fue del 86% y la especificidad del 100%. El protocolo basado en el plásmido unicopia SCAR220 se asoció a la menor sensibilidad (43%) pero a una especificidad del 100%. Los protocolos de PCR en tiempo real fueron muy reproducibles, sensibles y específicos. En ningún caso se detectaron resultados falsos positivos ni reacciones cruzadas. todos los laboratorios pudieron amplificar el ADN de H. capsulatum y las técnicas basadas en PCR en tiempo real parecen un instrumento prometedor para la detección eficiente de este patógeno en muestras clínicas." @default.
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• W1978391605 date "2013-10-01" @default.
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• W1978391605 title "Comparison of PCR protocols for detecting Histoplasma capsulatum DNA through a multicenter study" @default.
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• W1978391605 doi "https://doi.org/10.1016/j.riam.2013.03.004" @default.
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