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- W1978393385 abstract "This paper describes an analytical procedure for the quantitative determination of the pharmaceutical vehicle Cremophor EL in human plasma samples. The procedure is based on rapid binding of Coomassie brilliant blue G-250 to Cremophor EL following plasma protein precipitation with acetonitrile and analyte extraction withn-butylchloride. The binding of the dye to Cremophor EL causes a shift in the absorption maximum from 465 to 624 nm, which is monitored using an automated microplate-absorbance reader. The assay permits estimation of Cremophor EL concentrations in the range 0.05–1.00% (v/v) in 50 μL of human plasma, with percentage deviation and precision of ≤12 and ≤15%, respectively. The assay was subsequently used to measure Cremophor EL concentrations in plasma samples in support of a project to develop a pharmacokinetic model for this compound in patients receiving paclitaxel." @default.
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- W1978393385 date "1998-01-01" @default.
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- W1978393385 title "Quantitation of Cremophor EL in Human Plasma Samples Using a Colorimetric Dye-Binding Microassay" @default.
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- W1978393385 doi "https://doi.org/10.1006/abio.1997.2467" @default.
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