FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978397908> ?p ?o ?g. }

• W1978397908 endingPage "1996" @default.
• W1978397908 startingPage "1991" @default.
• W1978397908 abstract "Fluoroquinolone-resistant mutants of Mycobacterium smegmatis have been obtained in vitro by using ofloxacin as a selecting agent. Two types of mutants were identified according to their quinolone resistance patterns. Type 1 showed a low level of resistance to ofloxacin (MIC of 8 micrograms/ml), whereas a high level of resistance to this drug (MICs of 32 to 64 micrograms/ml) characterized type 2. By using two oligonucleotide primers homologous to DNA sequences flanking the quinolone resistance-determining region (QRDR) in the gyrA gene of Escherichia coli and Staphylococcus aureus, a 150-bp DNA fragment was obtained by PCR amplification from total DNA of two wild-type and five mutant strains of M. smegmatis. The nucleotide sequences of the amplified fragments were determined. The deduced amino acid sequence from the wild-type strains showed ca. 79% similarity with the QRDR in the gyrase A subunit from other gram-positive and gram-negative bacteria. The DNA sequences obtained from the fluoroquinolone-resistant mutants of M. smegmatis exhibited nucleotide modifications compared with the wild-type QRDR. The QRDR from type 1 mutants had a C-T or an A-G transition leading to a change from Ala-83 to Val or Asp-87 to Gly, respectively. The QRDR from type 2 mutants had a Val-83 mutation or both Val-83 and Gly-87 mutations detected in the type 1 mutants. These results suggest that point mutations in the QRDR of the mycobacterial gyrA gene are responsible for acquired quinolone resistance in M. smegmatis." @default.
• W1978397908 created "2016-06-24" @default.
• W1978397908 creator A5020574513 @default.
• W1978397908 creator A5055089481 @default.
• W1978397908 creator A5055642273 @default.
• W1978397908 creator A5082880299 @default.
• W1978397908 date "1994-09-01" @default.
• W1978397908 modified "2023-09-27" @default.
• W1978397908 title "Characterization of mutations in Mycobacterium smegmatis involved in resistance to fluoroquinolones" @default.
• W1978397908 cites W1566173303 @default.
• W1978397908 cites W1581614904 @default.
• W1978397908 cites W1845297809 @default.
• W1978397908 cites W1908882478 @default.
• W1978397908 cites W1962928558 @default.
• W1978397908 cites W1967457339 @default.
• W1978397908 cites W1969153299 @default.
• W1978397908 cites W1972676235 @default.
• W1978397908 cites W1977654774 @default.
• W1978397908 cites W1987310696 @default.
• W1978397908 cites W1996007131 @default.
• W1978397908 cites W2002486322 @default.
• W1978397908 cites W2009310436 @default.
• W1978397908 cites W2019306978 @default.
• W1978397908 cites W2029935652 @default.
• W1978397908 cites W2045868089 @default.
• W1978397908 cites W2047043554 @default.
• W1978397908 cites W2052467184 @default.
• W1978397908 cites W2054707817 @default.
• W1978397908 cites W2067146872 @default.
• W1978397908 cites W2072092253 @default.
• W1978397908 cites W2078772443 @default.
• W1978397908 cites W2146115441 @default.
• W1978397908 cites W2153547769 @default.
• W1978397908 cites W2156233609 @default.
• W1978397908 cites W2159463453 @default.
• W1978397908 cites W2159564238 @default.
• W1978397908 cites W2163628313 @default.
• W1978397908 cites W4235164085 @default.
• W1978397908 cites W4241841228 @default.
• W1978397908 doi "https://doi.org/10.1128/aac.38.9.1991" @default.
• W1978397908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/284673" @default.
• W1978397908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7811008" @default.
• W1978397908 hasPublicationYear "1994" @default.
• W1978397908 type Work @default.
• W1978397908 sameAs 1978397908 @default.
• W1978397908 citedByCount "33" @default.
• W1978397908 countsByYear W19783979082012 @default.
• W1978397908 countsByYear W19783979082014 @default.
• W1978397908 countsByYear W19783979082015 @default.
• W1978397908 crossrefType "journal-article" @default.
• W1978397908 hasAuthorship W1978397908A5020574513 @default.
• W1978397908 hasAuthorship W1978397908A5055089481 @default.
• W1978397908 hasAuthorship W1978397908A5055642273 @default.
• W1978397908 hasAuthorship W1978397908A5082880299 @default.
• W1978397908 hasBestOaLocation W19783979082 @default.
• W1978397908 hasConcept C104317684 @default.
• W1978397908 hasConcept C142724271 @default.
• W1978397908 hasConcept C143065580 @default.
• W1978397908 hasConcept C153911025 @default.
• W1978397908 hasConcept C167125095 @default.
• W1978397908 hasConcept C176944494 @default.
• W1978397908 hasConcept C207583985 @default.
• W1978397908 hasConcept C2777204207 @default.
• W1978397908 hasConcept C2777975735 @default.
• W1978397908 hasConcept C2778049240 @default.
• W1978397908 hasConcept C2778512257 @default.
• W1978397908 hasConcept C2779922871 @default.
• W1978397908 hasConcept C2781069245 @default.
• W1978397908 hasConcept C501593827 @default.
• W1978397908 hasConcept C54355233 @default.
• W1978397908 hasConcept C547475151 @default.
• W1978397908 hasConcept C71924100 @default.
• W1978397908 hasConcept C86803240 @default.
• W1978397908 hasConcept C89423630 @default.
• W1978397908 hasConceptScore W1978397908C104317684 @default.
• W1978397908 hasConceptScore W1978397908C142724271 @default.
• W1978397908 hasConceptScore W1978397908C143065580 @default.
• W1978397908 hasConceptScore W1978397908C153911025 @default.
• W1978397908 hasConceptScore W1978397908C167125095 @default.
• W1978397908 hasConceptScore W1978397908C176944494 @default.
• W1978397908 hasConceptScore W1978397908C207583985 @default.
• W1978397908 hasConceptScore W1978397908C2777204207 @default.
• W1978397908 hasConceptScore W1978397908C2777975735 @default.
• W1978397908 hasConceptScore W1978397908C2778049240 @default.
• W1978397908 hasConceptScore W1978397908C2778512257 @default.
• W1978397908 hasConceptScore W1978397908C2779922871 @default.
• W1978397908 hasConceptScore W1978397908C2781069245 @default.
• W1978397908 hasConceptScore W1978397908C501593827 @default.
• W1978397908 hasConceptScore W1978397908C54355233 @default.
• W1978397908 hasConceptScore W1978397908C547475151 @default.
• W1978397908 hasConceptScore W1978397908C71924100 @default.
• W1978397908 hasConceptScore W1978397908C86803240 @default.
• W1978397908 hasConceptScore W1978397908C89423630 @default.
• W1978397908 hasIssue "9" @default.
• W1978397908 hasLocation W19783979081 @default.
• W1978397908 hasLocation W19783979082 @default.
• W1978397908 hasLocation W19783979083 @default.
• W1978397908 hasOpenAccess W1978397908 @default.

• next