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- W1978398732 abstract "The quantification of neuropeptides may play a significant role in future drug development targeting central nervous system functions. Adequate method precision and accuracy is essential, and sample stability is an important factor. This study compares three sample preparation protocols and assesses the stability of targeted neuropeptides under standard laboratory conditions. The results show that the concentrations of substance P, dynorphin A, and calcitonin gene-related peptide (CGRP) change significantly in time when spinal cord tissues are homogenized in phosphate-buffered saline (PBS) buffer or PBS buffer containing a mammalian protease inhibitor cocktail but is stabilized when tissues are homogenized in a 0.25% trifluoroacetic acid solution." @default.
- W1978398732 created "2016-06-24" @default.
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- W1978398732 date "2010-12-01" @default.
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- W1978398732 title "Stability comparison between sample preparation procedures for mass spectrometry-based targeted or shotgun peptidomic analysis" @default.
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- W1978398732 doi "https://doi.org/10.1016/j.ab.2010.08.017" @default.
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