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• W1978414423 abstract "Aims: To identify, clone and sequence the iss (increased serum survival) gene from E. coli strain χ1378 isolated from Iranian poultry and to predict its protein product, Iss. Methods and Results: The iss gene from E. coli strain χ1378 was amplified and cloned into the pTZ57R/T vector and sequenced. From the DNA sequence, the Iss predictive protein was evaluated using bioinformatics. Iss from strain χ1378 had 100% identity with other E. coli serotypes and isolates from different origins and also 98% identity with E. coli O157:H7 Iss protein. Phylogenetic analysis showed no significant different phylogenic groups among E. coli strains. Conclusions: The strong association of predicted Iss protein among different E. coli strains suggests that it could be a good antigen to control and detect avian pathogenic E. coli (APEC). Significance and Impact of the study: Because the exact pathogenesis and the role of virulence factors are unknown, the Iss protein could be used as a target for vaccination in the future, but further research is required." @default.
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• W1978414423 date "2009-09-01" @default.
• W1978414423 modified "2023-09-25" @default.
• W1978414423 title "Identification, cloning and sequencing of<i>Escherichia coli</i>strain χ1378 (O78:K80)<i>iss</i>gene isolated from poultry colibacillosis in Iran" @default.
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• W1978414423 doi "https://doi.org/10.1111/j.1472-765x.2009.02681.x" @default.
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