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- W1978422034 abstract "• Metabolic control analysis allows the study of metabolic regulation. We applied both single- and double-manipulation top-down control analysis to examine the control of lipid accumulation in developing oilseed rape (Brassica napus) embryos. • The biosynthetic pathway was conceptually divided into two blocks of reactions (fatty acid biosynthesis (Block A), lipid assembly (Block B)) connected by a single system intermediate, the acyl-coenzyme A (acyl-CoA) pool. Single manipulation used exogenous oleate. Triclosan was used to inhibit specifically Block A, whereas diazepam selectively manipulated flux through Block B. • Exogenous oleate inhibited the radiolabelling of fatty acids from [1-14C]acetate, but stimulated that from [U-14C]glycerol into acyl lipids. The calculation of group flux control coefficients showed that c. 70% of the metabolic control was in the lipid assembly block of reactions. Monte Carlo simulations gave an estimation of the error of the resulting group flux control coefficients as 0.27 ± 0.06 for Block A and 0.73 ± 0.06 for Block B. • The two methods of control analysis gave very similar results and showed that Block B reactions were more important under our conditions. This contrasts notably with data from oil palm or olive fruit cultures and is important for efforts to increase oilseed rape lipid yields." @default.
- W1978422034 created "2016-06-24" @default.
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- W1978422034 date "2012-08-20" @default.
- W1978422034 modified "2023-10-10" @default.
- W1978422034 title "Metabolic control analysis of developing oilseed rape ( <i>Brassica napus</i> cv Westar) embryos shows that lipid assembly exerts significant control over oil accumulation" @default.
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- W1978422034 doi "https://doi.org/10.1111/j.1469-8137.2012.04262.x" @default.
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