Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978441008> ?p ?o ?g. }
- W1978441008 endingPage "34" @default.
- W1978441008 startingPage "28" @default.
- W1978441008 abstract "Development of antibiotic resistance may alter the virulence properties of bacterial organisms. In this study, nine clinical ceftriaxone-susceptible Salmonella enterica serotype Typhimurium strains were subjected to stepwise selection with increasing concentrations of ceftriaxone in culture media. Mutations in virulence-associated genes and antibiotic efflux genes were analyzed by polymerase chain reaction (PCR) and DNA sequencing. The expression levels of virulence genes invA and stn as well as efflux pump genes tolC, arcA, and arcB before and after the selection were measured by real-time quantitative reverse transcription–polymerase chain reaction (qRT-PCR). The stepwise selection resulted in the development of Salmonella strains that were highly resistant to ceftriaxone. Sequence analysis did not reveal any mutations or deletions in the examined virulence genes and regulatory gene, but a silent mutation (T423C) in acrR (encoding a repressor for the efflux pump) was detected in most of the ceftriaxone-resistant strains. The qRT-PCR revealed increased expression of the AcrAB-TolC efflux pump and decreased expression of invA and stn in the ceftriaxone-resistant strains. Moreover, decreased invasion into cultured epithelial cells and reduced growth rates were observed with the resistant strains. These results suggest that acquisition of ceftriaxone resistance is associated with the overexpression of the AcrAB-TolC efflux pump and leads to reduced virulence in Salmonella Typhimurium." @default.
- W1978441008 created "2016-06-24" @default.
- W1978441008 creator A5015166166 @default.
- W1978441008 creator A5034874059 @default.
- W1978441008 creator A5039647837 @default.
- W1978441008 creator A5047438925 @default.
- W1978441008 creator A5051097578 @default.
- W1978441008 creator A5064564309 @default.
- W1978441008 creator A5064992427 @default.
- W1978441008 creator A5075518169 @default.
- W1978441008 creator A5079703386 @default.
- W1978441008 date "2013-01-01" @default.
- W1978441008 modified "2023-10-18" @default.
- W1978441008 title "Development of Ceftriaxone Resistance Affects the Virulence Properties ofSalmonella entericaSerotype Typhimurium Strains" @default.
- W1978441008 cites W1553543524 @default.
- W1978441008 cites W1584888890 @default.
- W1978441008 cites W1875628381 @default.
- W1978441008 cites W1973261412 @default.
- W1978441008 cites W1973714359 @default.
- W1978441008 cites W1974702731 @default.
- W1978441008 cites W2003021105 @default.
- W1978441008 cites W2009471277 @default.
- W1978441008 cites W2012218328 @default.
- W1978441008 cites W2035313213 @default.
- W1978441008 cites W2042139721 @default.
- W1978441008 cites W2046170552 @default.
- W1978441008 cites W2076233570 @default.
- W1978441008 cites W2077668745 @default.
- W1978441008 cites W2085456089 @default.
- W1978441008 cites W2085565803 @default.
- W1978441008 cites W2089239558 @default.
- W1978441008 cites W2096396131 @default.
- W1978441008 cites W2096682277 @default.
- W1978441008 cites W2103394403 @default.
- W1978441008 cites W2103592709 @default.
- W1978441008 cites W2104620113 @default.
- W1978441008 cites W2107277218 @default.
- W1978441008 cites W2114626239 @default.
- W1978441008 cites W2115490464 @default.
- W1978441008 cites W2117249826 @default.
- W1978441008 cites W2122820326 @default.
- W1978441008 cites W2128274513 @default.
- W1978441008 cites W2130376844 @default.
- W1978441008 cites W2132320990 @default.
- W1978441008 cites W2135173569 @default.
- W1978441008 cites W2139790819 @default.
- W1978441008 cites W2156449112 @default.
- W1978441008 cites W2164863538 @default.
- W1978441008 cites W2166826452 @default.
- W1978441008 cites W2172097617 @default.
- W1978441008 cites W4243575454 @default.
- W1978441008 doi "https://doi.org/10.1089/fpd.2012.1216" @default.
- W1978441008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23320420" @default.
- W1978441008 hasPublicationYear "2013" @default.
- W1978441008 type Work @default.
- W1978441008 sameAs 1978441008 @default.
- W1978441008 citedByCount "7" @default.
- W1978441008 countsByYear W19784410082015 @default.
- W1978441008 countsByYear W19784410082016 @default.
- W1978441008 countsByYear W19784410082019 @default.
- W1978441008 countsByYear W19784410082021 @default.
- W1978441008 countsByYear W19784410082023 @default.
- W1978441008 crossrefType "journal-article" @default.
- W1978441008 hasAuthorship W1978441008A5015166166 @default.
- W1978441008 hasAuthorship W1978441008A5034874059 @default.
- W1978441008 hasAuthorship W1978441008A5039647837 @default.
- W1978441008 hasAuthorship W1978441008A5047438925 @default.
- W1978441008 hasAuthorship W1978441008A5051097578 @default.
- W1978441008 hasAuthorship W1978441008A5064564309 @default.
- W1978441008 hasAuthorship W1978441008A5064992427 @default.
- W1978441008 hasAuthorship W1978441008A5075518169 @default.
- W1978441008 hasAuthorship W1978441008A5079703386 @default.
- W1978441008 hasConcept C104317684 @default.
- W1978441008 hasConcept C200082930 @default.
- W1978441008 hasConcept C2776460866 @default.
- W1978441008 hasConcept C2776520383 @default.
- W1978441008 hasConcept C2779607309 @default.
- W1978441008 hasConcept C2781065037 @default.
- W1978441008 hasConcept C49105822 @default.
- W1978441008 hasConcept C501593827 @default.
- W1978441008 hasConcept C523546767 @default.
- W1978441008 hasConcept C54355233 @default.
- W1978441008 hasConcept C60987743 @default.
- W1978441008 hasConcept C86803240 @default.
- W1978441008 hasConcept C89423630 @default.
- W1978441008 hasConcept C94665300 @default.
- W1978441008 hasConceptScore W1978441008C104317684 @default.
- W1978441008 hasConceptScore W1978441008C200082930 @default.
- W1978441008 hasConceptScore W1978441008C2776460866 @default.
- W1978441008 hasConceptScore W1978441008C2776520383 @default.
- W1978441008 hasConceptScore W1978441008C2779607309 @default.
- W1978441008 hasConceptScore W1978441008C2781065037 @default.
- W1978441008 hasConceptScore W1978441008C49105822 @default.
- W1978441008 hasConceptScore W1978441008C501593827 @default.
- W1978441008 hasConceptScore W1978441008C523546767 @default.
- W1978441008 hasConceptScore W1978441008C54355233 @default.
- W1978441008 hasConceptScore W1978441008C60987743 @default.
- W1978441008 hasConceptScore W1978441008C86803240 @default.