Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978448581> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1978448581 endingPage "16" @default.
- W1978448581 startingPage "9" @default.
- W1978448581 abstract "Barnase is a potent ribonuclease widely used as a cytotoxic agent, tightly regulated by barstar to maintain cell viability. In this report, we describe a new composite regulatory system to control barnase cytotoxicity and expression, involving barstar and lacI genes under control of the NifA-, sigma54-dependent Sinorhizobium meliloti nifH promoter, and the barnase gene under control of the LacI-repressible ptac promoter. In this system, expression of thenifH promoter, activated by constitutively expressed NifA, resulted in constitutive synthesis of the LacI and barstar proteins. LacI, in turn, represses transcription of the barnase gene and barstar inhibits any ribonuclease activity. Full expression of the barnase gene induced by IPTG led to cell death. Control of barnase synthesis and activity could be achieved by regulating nifA expression and NifA protein activity by specific environmental signals." @default.
- W1978448581 created "2016-06-24" @default.
- W1978448581 creator A5001284090 @default.
- W1978448581 creator A5012168800 @default.
- W1978448581 creator A5047519860 @default.
- W1978448581 creator A5085180904 @default.
- W1978448581 date "2005-07-01" @default.
- W1978448581 modified "2023-10-18" @default.
- W1978448581 title "A new system to control the barnase expression by a NifA-dependent promoter" @default.
- W1978448581 cites W1512899354 @default.
- W1978448581 cites W1849544275 @default.
- W1978448581 cites W1963940836 @default.
- W1978448581 cites W1965859342 @default.
- W1978448581 cites W1978862919 @default.
- W1978448581 cites W1985724808 @default.
- W1978448581 cites W1996030570 @default.
- W1978448581 cites W2016823281 @default.
- W1978448581 cites W2029841573 @default.
- W1978448581 cites W2044811703 @default.
- W1978448581 cites W2049045389 @default.
- W1978448581 cites W2054737259 @default.
- W1978448581 cites W2077777987 @default.
- W1978448581 cites W2085793386 @default.
- W1978448581 cites W2099559757 @default.
- W1978448581 cites W2136914000 @default.
- W1978448581 cites W2150761944 @default.
- W1978448581 cites W2151009382 @default.
- W1978448581 cites W2163638804 @default.
- W1978448581 cites W4255914531 @default.
- W1978448581 doi "https://doi.org/10.1016/j.jbiotec.2005.03.002" @default.
- W1978448581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15908028" @default.
- W1978448581 hasPublicationYear "2005" @default.
- W1978448581 type Work @default.
- W1978448581 sameAs 1978448581 @default.
- W1978448581 citedByCount "9" @default.
- W1978448581 countsByYear W19784485812013 @default.
- W1978448581 countsByYear W19784485812014 @default.
- W1978448581 countsByYear W19784485812017 @default.
- W1978448581 countsByYear W19784485812018 @default.
- W1978448581 crossrefType "journal-article" @default.
- W1978448581 hasAuthorship W1978448581A5001284090 @default.
- W1978448581 hasAuthorship W1978448581A5012168800 @default.
- W1978448581 hasAuthorship W1978448581A5047519860 @default.
- W1978448581 hasAuthorship W1978448581A5085180904 @default.
- W1978448581 hasConcept C101762097 @default.
- W1978448581 hasConcept C104317684 @default.
- W1978448581 hasConcept C113629113 @default.
- W1978448581 hasConcept C138885662 @default.
- W1978448581 hasConcept C150194340 @default.
- W1978448581 hasConcept C153911025 @default.
- W1978448581 hasConcept C170344550 @default.
- W1978448581 hasConcept C179926584 @default.
- W1978448581 hasConcept C185592680 @default.
- W1978448581 hasConcept C2776559745 @default.
- W1978448581 hasConcept C2778976237 @default.
- W1978448581 hasConcept C41895202 @default.
- W1978448581 hasConcept C54355233 @default.
- W1978448581 hasConcept C67705224 @default.
- W1978448581 hasConcept C86803240 @default.
- W1978448581 hasConcept C95444343 @default.
- W1978448581 hasConceptScore W1978448581C101762097 @default.
- W1978448581 hasConceptScore W1978448581C104317684 @default.
- W1978448581 hasConceptScore W1978448581C113629113 @default.
- W1978448581 hasConceptScore W1978448581C138885662 @default.
- W1978448581 hasConceptScore W1978448581C150194340 @default.
- W1978448581 hasConceptScore W1978448581C153911025 @default.
- W1978448581 hasConceptScore W1978448581C170344550 @default.
- W1978448581 hasConceptScore W1978448581C179926584 @default.
- W1978448581 hasConceptScore W1978448581C185592680 @default.
- W1978448581 hasConceptScore W1978448581C2776559745 @default.
- W1978448581 hasConceptScore W1978448581C2778976237 @default.
- W1978448581 hasConceptScore W1978448581C41895202 @default.
- W1978448581 hasConceptScore W1978448581C54355233 @default.
- W1978448581 hasConceptScore W1978448581C67705224 @default.
- W1978448581 hasConceptScore W1978448581C86803240 @default.
- W1978448581 hasConceptScore W1978448581C95444343 @default.
- W1978448581 hasIssue "1" @default.
- W1978448581 hasLocation W19784485811 @default.
- W1978448581 hasLocation W19784485812 @default.
- W1978448581 hasOpenAccess W1978448581 @default.
- W1978448581 hasPrimaryLocation W19784485811 @default.
- W1978448581 hasRelatedWork W2023905906 @default.
- W1978448581 hasRelatedWork W2058303636 @default.
- W1978448581 hasRelatedWork W2065022193 @default.
- W1978448581 hasRelatedWork W2152116863 @default.
- W1978448581 hasRelatedWork W2410256846 @default.
- W1978448581 hasRelatedWork W2515895007 @default.
- W1978448581 hasRelatedWork W2811441309 @default.
- W1978448581 hasRelatedWork W3049265919 @default.
- W1978448581 hasRelatedWork W3126354933 @default.
- W1978448581 hasRelatedWork W66335451 @default.
- W1978448581 hasVolume "118" @default.
- W1978448581 isParatext "false" @default.
- W1978448581 isRetracted "false" @default.
- W1978448581 magId "1978448581" @default.
- W1978448581 workType "article" @default.