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- W1978457906 abstract "1. The aim of the present study was to investigate noradrenaline (NA)-induced regulation of α1-adrenoceptor (AR) mRNA expression in human embryonic kidney (HEK) 293 cells stably expressing cloned α1-AR subtypes with similar receptor densities. Stable transfection was performed by calcium phosphate precipitation. Receptor expression was detected by radioligand binding assay. The mRNA expression was measured by RNase protection assay. 2. α1-Adrenoceptor subtype mRNA respond in distinct ways following prolonged exposure to NA. The mRNA level of the α1A-AR subtype was unchanged, the mRNA level of the α1B-AR subtype was increased and the mRNA level of the α1D-AR subtype declined time dependently. The protein kinase C (PKC) inhibitor calphostin C or RO 31-8220 abolished the NA-induced downregulation of α1D-AR mRNA. Phorbol myristate acetate (PMA), a PKC activator, similarly repressed the effects of NA on α1D-AR. However, calphostin C, RO 31-8220 or PMA had no effect on the induction of α1B-AR mRNA by NA. The Ca2+-ATPase inhibitor thapsigargin or the calcium chelator 1,2-bis-(o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM) had no effect on the repression of α1D-AR mRNA, but did inhibit the induction of α1B-AR mRNA by NA. Noradrenaline significantly decelerated the degradation of α1B-AR mRNA, but had no effect on the degradation of α1D-AR mRNA. 3. Thus, the mRNA expression of three α1-AR subtypes in HEK293 cells is differentially regulated through distinct signal transduction pathways under sustained NA stimulation. The upregulation of α1B-AR mRNA is via the Ca2+ pathway, whereas the downregulation of α1D-AR mRNA is via the PKC pathway." @default.
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- W1978457906 date "2002-12-01" @default.
- W1978457906 modified "2023-09-26" @default.
- W1978457906 title "Changes in mRNA expression induced by sustained noradrenaline stimulation are different for alpha1-adrenoceptor subtypes in HEK293 cells" @default.
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- W1978457906 doi "https://doi.org/10.1046/j.1440-1681.2002.03775.x" @default.
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