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- W1978492020 abstract "We report a fluorescence-based assay for measuring the affinity of microtubule binding proteins for microtubules. The affinity of any fluorescently tagged protein for taxol-stabilized microtubules can be measured with this assay. We describe the assay and provide a detailed protocol. Using this assay, we found that the affinity of the Dam1 complex for microtubules is decreased by the presence of free unpolymerized tubulin and is sensitive to the salt concentration in the binding buffer. These effects may account for the previous differences in binding affinities reported." @default.
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- W1978492020 date "2011-03-01" @default.
- W1978492020 modified "2023-09-26" @default.
- W1978492020 title "An assay to measure the affinity of proteins for microtubules by quantitative fluorescent microscopy" @default.
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- W1978492020 doi "https://doi.org/10.1016/j.ab.2010.12.009" @default.
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