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• W1978496762 startingPage "1301" @default.
• W1978496762 abstract "The locus ceruleus (LC) contains neurons that increase their firing rate (FR) in vitro when exposed to elevated CO 2 /H + and have been proposed to influence the respiratory network to make compensatory adjustments in ventilation. Prp57 transgenic mice express green fluorescent protein (GFP) in the LC and were used to isolate, culture, and target LC neurons for electrophysiological recording. We hypothesized that GFP-LC neurons would exhibit CO 2 /H + chemosensitivity under primary culture conditions, evidenced as a change in FR. This is the first study to quantify CO 2 /H + responses in LC neuron FR in cell culture. Neurons were continuously bathed with solutions containing antagonists of glutamate and GABA receptors, and the acid-base status was changed from control (5% CO 2 ; pH ∼7.4) to hypercapnic acidosis (9% CO 2 ; pH ∼7.2) and hypocapnic alkalosis (3% CO 2 ; pH ∼7.6). FR was quantified during perforated patch current clamp recordings. Approximately 86% of GFP-LC neurons were stimulated, and ∼14% were insensitive to changes in CO 2 /H + . The magnitude of the response of these neurons depended on the baseline FR, ranging from 155.9 ± 6% when FR started at 2.95 ± 0.49 Hz to 381 ± 55.6% when FR started at 1.32 ± 0.31 Hz. These results demonstrate that cultured LC neurons from Prp57 transgenic mice retain functional sensing molecules necessary for CO 2 /H + responses. Prp57 transgenic mice will serve as a valuable model to delineate mechanisms involved in CO 2 /H + responsiveness in catecholaminergic neurons." @default.
• W1978496762 created "2016-06-24" @default.
• W1978496762 creator A5019717439 @default.
• W1978496762 creator A5037071752 @default.
• W1978496762 creator A5082712234 @default.
• W1978496762 date "2008-10-01" @default.
• W1978496762 modified "2023-10-15" @default.
• W1978496762 title "GFP-expressing locus ceruleus neurons from Prp57 transgenic mice exhibit CO₂/H⁺ responses in primary cell culture" @default.
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• W1978496762 doi "https://doi.org/10.1152/japplphysiol.90414.2008" @default.
• W1978496762 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2576037" @default.
• W1978496762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18635881" @default.
• W1978496762 hasPublicationYear "2008" @default.
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