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• W1978527502 abstract "Cytotoxicity of cadmium-containing silica nanoparticles Cd-SiO 2 NPs (0.05–100 µg/mL) versus SiO 2 NPs and CdCl 2 was evaluated by an in vitro test battery in A549 by assessing (i) mitochondrial function, (ii) membrane integrity/cell morphology, (iii) cell growth/proliferation, (iv) apoptotic pathway, (v) oxidative stress, after short- (24–48 h) and long-term (10 days) exposure. Both Cd-SiO 2 NPs and CdCl 2 produced dose-dependent cytotoxic effects: (i) MTT-assay : similar cytotoxicity pattern was observed at both 24 and 48 h, with a more Cd-SiO 2 NPs pronounced effect than CdCl 2 . Cd-SiO 2 NPs induced mortality (about 50%) at 1 μ g/mL, CdCl 2 at 25 μ g/mL; (ii) calcein-AM/PI staining : decrease in cell viability, noticeable at 25 μ g/mL, enhanced markedly at 50 and 100 μ g/mL, after 24 h. Cd-SiO 2 NPs induced higher mortality than CdCl 2 (25% versus 4%, resp., at 25 μ g/mL) with further exacerbation after 48h; (iii) clonogenic assay : exposure for longer period (10 days) compromised the A549 proliferative capacity at very low dose (0.05 μ g/mL); (iv) a progressive activation of caspase-3 immunolabelling was detected already at 1 μ g/mL; (v) GSH intracellular level was modified by all compounds. In summary, in vitro data demonstrated that both Cd-SiO 2 NPs and CdCl 2 affected all investigated endpoints, more markedly after Cd-SiO 2 NPs, while SiO 2 NPs influenced GSH only." @default.
• W1978527502 created "2016-06-24" @default.
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• W1978527502 date "2013-01-01" @default.
• W1978527502 modified "2023-09-27" @default.
• W1978527502 title "<i>In Vitro</i>Toxicity Evaluation of Engineered Cadmium-Coated Silica Nanoparticles on Human Pulmonary Cells" @default.
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• W1978527502 doi "https://doi.org/10.1155/2013/931785" @default.
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