FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978532813> ?p ?o ?g. }

• W1978532813 endingPage "e29277" @default.
• W1978532813 startingPage "e29277" @default.
• W1978532813 abstract "Background Listeria monocytogenes, an intracellular foodborne pathogen, infects immunocompromised hosts. The primary route of transmission is through contaminated food. In the gastrointestinal tract, it traverses the epithelial barrier through intracellular or paracellular routes. Strategies to prevent L. monocytogenes entry can potentially minimize infection in high-risk populations. Listeria adhesion protein (LAP) aids L. monocytogenes in crossing epithelial barriers via the paracellular route. The use of recombinant probiotic bacteria expressing LAP would aid targeted clearance of Listeria from the gut and protect high-risk populations from infection. Methodology/Principal Findings The objective was to investigate the ability of probiotic bacteria or LAP-expressing recombinant probiotic Lactobacillus paracasei (LbpLAP) to prevent L. monocytogenes adhesion, invasion, and transwell-based transepithelial translocation in a Caco-2 cell culture model. Several wild type probiotic bacteria showed strong adhesion to Caco-2 cells but none effectively prevented L. monocytogenes infection. Pre-exposure to LbpLAP for 1, 4, 15, or 24 h significantly (P<0.05) reduced adhesion, invasion, and transepithelial translocation of L. monocytogenes in Caco-2 cells, whereas pre-exposure to parental Lb. paracasei had no significant effect. Similarly, LbpLAP pre-exposure reduced L. monocytogenes translocation by as much as 46% after 24 h. LbpLAP also prevented L. monocytogenes-mediated cell damage and compromise of tight junction integrity. Furthermore, LbpLAP cells reduced L. monocytogenes-mediated cell cytotoxicity by 99.8% after 1 h and 79% after 24 h. Conclusions/Significance Wild type probiotic bacteria were unable to prevent L. monocytogenes infection in vitro. In contrast, LbpLAP blocked adhesion, invasion, and translocation of L. monocytogenes by interacting with host cell receptor Hsp60, thereby protecting cells from infection. These data show promise for the use of recombinant probiotics in preventing L. monocytogenes infection in high-risk populations." @default.
• W1978532813 created "2016-06-24" @default.
• W1978532813 creator A5000790147 @default.
• W1978532813 creator A5052873020 @default.
• W1978532813 creator A5069928595 @default.
• W1978532813 date "2012-01-03" @default.
• W1978532813 modified "2023-10-18" @default.
• W1978532813 title "Recombinant Probiotic Expressing Listeria Adhesion Protein Attenuates Listeria monocytogenes Virulence In Vitro" @default.
• W1978532813 cites W1601070312 @default.
• W1978532813 cites W1602115295 @default.
• W1978532813 cites W167488892 @default.
• W1978532813 cites W190518456 @default.
• W1978532813 cites W1909750055 @default.
• W1978532813 cites W1964263490 @default.
• W1978532813 cites W1965202556 @default.
• W1978532813 cites W1966917612 @default.
• W1978532813 cites W1971280478 @default.
• W1978532813 cites W1972468032 @default.
• W1978532813 cites W1974674463 @default.
• W1978532813 cites W1975167841 @default.
• W1978532813 cites W1977337243 @default.
• W1978532813 cites W1979717620 @default.
• W1978532813 cites W1982072530 @default.
• W1978532813 cites W1986360956 @default.
• W1978532813 cites W1987838634 @default.
• W1978532813 cites W1993805426 @default.
• W1978532813 cites W1993824769 @default.
• W1978532813 cites W1997468258 @default.
• W1978532813 cites W1998963977 @default.
• W1978532813 cites W2002995292 @default.
• W1978532813 cites W2003168897 @default.
• W1978532813 cites W2003688768 @default.
• W1978532813 cites W2003705537 @default.
• W1978532813 cites W2004986356 @default.
• W1978532813 cites W2012412543 @default.
• W1978532813 cites W2013682169 @default.
• W1978532813 cites W2016210901 @default.
• W1978532813 cites W2017524802 @default.
• W1978532813 cites W2021872061 @default.
• W1978532813 cites W2033001457 @default.
• W1978532813 cites W2033507994 @default.
• W1978532813 cites W2038273124 @default.
• W1978532813 cites W2039079048 @default.
• W1978532813 cites W2046417457 @default.
• W1978532813 cites W2047909468 @default.
• W1978532813 cites W2059964548 @default.
• W1978532813 cites W2064316722 @default.
• W1978532813 cites W2064654077 @default.
• W1978532813 cites W2072004656 @default.
• W1978532813 cites W2073395843 @default.
• W1978532813 cites W2074513558 @default.
• W1978532813 cites W2080272180 @default.
• W1978532813 cites W2081536852 @default.
• W1978532813 cites W2083484540 @default.
• W1978532813 cites W2090084901 @default.
• W1978532813 cites W2093774099 @default.
• W1978532813 cites W2096011212 @default.
• W1978532813 cites W2097480342 @default.
• W1978532813 cites W2101413510 @default.
• W1978532813 cites W2106062601 @default.
• W1978532813 cites W2110007447 @default.
• W1978532813 cites W2112281734 @default.
• W1978532813 cites W2113379278 @default.
• W1978532813 cites W2115045522 @default.
• W1978532813 cites W2116839692 @default.
• W1978532813 cites W2116948846 @default.
• W1978532813 cites W2126569085 @default.
• W1978532813 cites W2128591134 @default.
• W1978532813 cites W2129513838 @default.
• W1978532813 cites W2134724901 @default.
• W1978532813 cites W2135866777 @default.
• W1978532813 cites W2137907845 @default.
• W1978532813 cites W2139239302 @default.
• W1978532813 cites W2141542812 @default.
• W1978532813 cites W2144970033 @default.
• W1978532813 cites W2145189896 @default.
• W1978532813 cites W2148516418 @default.
• W1978532813 cites W2149190469 @default.
• W1978532813 cites W2153727850 @default.
• W1978532813 cites W2159790343 @default.
• W1978532813 cites W2162397387 @default.
• W1978532813 cites W2163237429 @default.
• W1978532813 cites W2163689273 @default.
• W1978532813 cites W2169568623 @default.
• W1978532813 cites W22758001 @default.
• W1978532813 doi "https://doi.org/10.1371/journal.pone.0029277" @default.
• W1978532813 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3250429" @default.
• W1978532813 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22235279" @default.
• W1978532813 hasPublicationYear "2012" @default.
• W1978532813 type Work @default.
• W1978532813 sameAs 1978532813 @default.
• W1978532813 citedByCount "83" @default.
• W1978532813 countsByYear W19785328132012 @default.
• W1978532813 countsByYear W19785328132013 @default.
• W1978532813 countsByYear W19785328132014 @default.
• W1978532813 countsByYear W19785328132015 @default.
• W1978532813 countsByYear W19785328132016 @default.
• W1978532813 countsByYear W19785328132017 @default.

• next