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- W1978534965 abstract "The transverse location normal to the bilayer surface of a series of n-(9-anthroyloxy) fatty acid probes, where n = 2, 3, 6, 7, 9, 12, and 16, was determined by fluorescence quenching measurements with a flow cytometer. We show that the anthroyloxy moieties of the probes locate at a graded series of depths in the outer leaflet of the plasma membrane of living HeLa cells, in a manner similar to that previously observed for model membrane systems, and mitochondria. For different n, the efficiency of quenching with an aqueous phase quencher, Cu+2, was 2 greater than or equal to 3 greater than 6 greater than or equal to 7 greater than 9 greater than 12 greater than 16. Therefore, flow cytometry permits use of these probes for measurements of dynamic parameters related to membrane fluidity at different depths in the plasma membranes of living cells." @default.
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- W1978534965 date "1991-01-01" @default.
- W1978534965 modified "2023-09-23" @default.
- W1978534965 title "Fluorescence quenching of a series of membrane probes measured in living cells by flow cytometry" @default.
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- W1978534965 doi "https://doi.org/10.1002/cyto.990120307" @default.
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