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• W1978540379 abstract "Single-stranded DNA molecules containing clustered G-repeats can be assembled into various four-stranded structures linked by G-quartets. Here, we report that such molecules can also drive the assembly of other DNA molecules containing G-repeats into specific four-stranded structures. In these assays, the oligonucleotides 5′-CAGGCTGAGCAGGTAC GGGGG AGCT GGGG TAGATTGGAATGTAG-3′ (oligo D) and 5′-C GGGGG AGCT GGGG T-3′ (oligo B), consisting of sequences found in immunoglobulin switch regions, were annealed in a buffer containing K+ and the annealing products were analyzed by polyacrylamide gel electrophoresis. This analysis revealed that whereas annealing of each oligo alone produced four-stranded structures designated D2 and B2, annealing of mixtures containing both oligos produced additional complexes designated D2∗ and B2∗. D2∗ and B2∗ were found to contain only D molecules and only B molecules, respectively. The yield of D2∗ increased and the yield of 2∗ decreased, as the concentration ratio oligo B/oligo D was increased. These results indicated that B can drive the assembly of D into D2∗ and D can drive the assembly of B into B2∗. Further studies revealed that while the assembly of D2 followed a second order kinetics, the B-driven assembly of D2∗ followed a first order kinetics. Dimethyl sulfate footprinting indicated that both D2and D2∗ are four-stranded structures containing two parallel and two antiparallel chains. In addition, annealing of D mixed with various B mutants showed that only mutants containing two G-clusters can drive the assembly of D2∗. Based on these data, we propose that in the process of D2∗ assembly, a four-stranded intermediate containing B and D is formed and then dissociates into D2∗ and B in a rate-limiting first order reaction. Driver mechanisms of this type may cause formation of specific four-stranded structures at G-rich chromosomal sites, thereby regulating processes such as recombination and telomere synthesis." @default.
• W1978540379 created "2016-06-24" @default.
• W1978540379 creator A5008678260 @default.
• W1978540379 creator A5019079423 @default.
• W1978540379 creator A5088004179 @default.
• W1978540379 date "1999-02-01" @default.
• W1978540379 modified "2023-10-16" @default.
• W1978540379 title "DNA molecules can drive the assembly of other DNA molecules into specific four-stranded structures" @default.
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• W1978540379 doi "https://doi.org/10.1006/jmbi.1998.2461" @default.
• W1978540379 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9931248" @default.
• W1978540379 hasPublicationYear "1999" @default.
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