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- W1978550059 abstract "Lysyl-tRNA synthetases are synthesized from two distinct genes in E coli, lysS and lysU, but neither gene product has been purified distinctively by using overproducing systems. The lysS gene has been identified by a herC mutation which restores maintenance of the mutant ColE1 replicon. The herC gene product was overproduced by using a tac promoter fusion and purified to homogeneity. The purified HerC protein possesses a lysyl-tRNA synthetase activity as predicted by the sequence identity of herC to lysS. The procedure is useful for rapid mass-scale purification of lysyl-tRNA synthetase." @default.
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- W1978550059 date "1992-06-01" @default.
- W1978550059 modified "2023-09-27" @default.
- W1978550059 title "Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of E coli" @default.
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- W1978550059 doi "https://doi.org/10.1016/0300-9084(92)90158-b" @default.
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