FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978562493> ?p ?o ?g. }

• W1978562493 endingPage "141" @default.
• W1978562493 startingPage "134" @default.
• W1978562493 abstract "Abstract Background Apparently normal rat liver epithelial cells (WB‐F344) have been widely used in studies pertaining to carcinogenesis. Ionizing radiation, a well known carcinogen, is known to perturb cell‐cycle progression in a dose‐dependent manner, thereby causing delay in cell proliferation. However, for WB‐F344 cells, there is a paucity of such data, which are of substantial importance in understanding their radiation response. Here, the distribution of phases in the cell‐cycle and the proliferation ability of WB‐F344 cells are characterized at various time points after the cells have been irradiated with different doses of γ‐rays. Methods After WB‐F344 cells reached 100% confluence, they were trypsinized and suspended at 3.5 × 10 5 cells/ml in culture medium. Cells were irradiated in suspension with 137 Cs γ‐rays at doses from 1–10 Gy. After irradiation, 1 × 10 5 cells were plated into 60 × 15‐mm culture dishes and incubated at 37°C, with 2% CO 2 and 98% air. At 12, 24, 36, 48, and 60 h postirradiation, cells were harvested, counted, and subjected to flow cytometric cell‐cycle analysis. Results Growth curves of WB‐F344 cells irradiated with γ‐rays started to separate at 36 h postirradiation. By 60 h postirradiation, the growth curves for each of the 10 absorbed doses were distinctly separated. Drastic redistributions of control and irradiated cells within G 0 /G 1 ‐, S‐, and G 2 /M‐phases of the cell cycle were observed during the first 36 h of cell growth. At each time point postirradiation, cell‐cycle phase profiles of irradiated cells were altered in a dose‐dependent manner. In general, there was a strong correlation between the percentage of G 2 /M‐phase cells and absorbed dose, with the exception of 24 h postirradiation. The percentage of G 2 /M‐phase cells increased as a function of time postirradiation, suggestive of delays in the passage of cells through the G 2 cell‐cycle checkpoint. Conclusions This work provides a general description of cell cycle redistribution and repopulation kinetics of WB‐F344 cells at various times postirradiation of quiescent cells that were subsequently allowed to proliferate. In general, growth inhibition and delays in progression through G 2 /M‐phase correlated well with radiation dose. These data should be of considerable significance in the design of experiments that examine the radiation response of these cells. © 2004 Wiley‐Liss, Inc." @default.
• W1978562493 created "2016-06-24" @default.
• W1978562493 creator A5007527909 @default.
• W1978562493 creator A5021264034 @default.
• W1978562493 creator A5071465211 @default.
• W1978562493 date "2004-08-26" @default.
• W1978562493 modified "2023-10-17" @default.
• W1978562493 title "Characterization of cell-cycle progression and growth of WB-F344 normal rat liver epithelial cells following gamma-ray exposure" @default.
• W1978562493 cites W168276155 @default.
• W1978562493 cites W1970028346 @default.
• W1978562493 cites W1972487225 @default.
• W1978562493 cites W1981997467 @default.
• W1978562493 cites W1996908651 @default.
• W1978562493 cites W2002599698 @default.
• W1978562493 cites W2005354143 @default.
• W1978562493 cites W2017927228 @default.
• W1978562493 cites W2018521345 @default.
• W1978562493 cites W2027321340 @default.
• W1978562493 cites W2036754489 @default.
• W1978562493 cites W2037399198 @default.
• W1978562493 cites W2039142844 @default.
• W1978562493 cites W2041444090 @default.
• W1978562493 cites W2046374181 @default.
• W1978562493 cites W2050826842 @default.
• W1978562493 cites W2073728593 @default.
• W1978562493 cites W2075106638 @default.
• W1978562493 cites W2075497030 @default.
• W1978562493 cites W2092245345 @default.
• W1978562493 cites W2160483159 @default.
• W1978562493 cites W2161680599 @default.
• W1978562493 cites W2162135540 @default.
• W1978562493 cites W279787071 @default.
• W1978562493 cites W4212887994 @default.
• W1978562493 cites W4298223063 @default.
• W1978562493 doi "https://doi.org/10.1002/cyto.a.20065" @default.
• W1978562493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15382025" @default.
• W1978562493 hasPublicationYear "2004" @default.
• W1978562493 type Work @default.
• W1978562493 sameAs 1978562493 @default.
• W1978562493 citedByCount "11" @default.
• W1978562493 countsByYear W19785624932012 @default.
• W1978562493 countsByYear W19785624932016 @default.
• W1978562493 countsByYear W19785624932018 @default.
• W1978562493 countsByYear W19785624932019 @default.
• W1978562493 countsByYear W19785624932022 @default.
• W1978562493 crossrefType "journal-article" @default.
• W1978562493 hasAuthorship W1978562493A5007527909 @default.
• W1978562493 hasAuthorship W1978562493A5021264034 @default.
• W1978562493 hasAuthorship W1978562493A5071465211 @default.
• W1978562493 hasConcept C104317684 @default.
• W1978562493 hasConcept C111337013 @default.
• W1978562493 hasConcept C114246631 @default.
• W1978562493 hasConcept C117262875 @default.
• W1978562493 hasConcept C121332964 @default.
• W1978562493 hasConcept C1491633281 @default.
• W1978562493 hasConcept C153911025 @default.
• W1978562493 hasConcept C181199279 @default.
• W1978562493 hasConcept C18231593 @default.
• W1978562493 hasConcept C185544564 @default.
• W1978562493 hasConcept C185592680 @default.
• W1978562493 hasConcept C2583947 @default.
• W1978562493 hasConcept C2780340462 @default.
• W1978562493 hasConcept C29537977 @default.
• W1978562493 hasConcept C38240268 @default.
• W1978562493 hasConcept C54355233 @default.
• W1978562493 hasConcept C553184892 @default.
• W1978562493 hasConcept C55493867 @default.
• W1978562493 hasConcept C555283112 @default.
• W1978562493 hasConcept C62112901 @default.
• W1978562493 hasConcept C81885089 @default.
• W1978562493 hasConcept C85813293 @default.
• W1978562493 hasConcept C86803240 @default.
• W1978562493 hasConcept C95444343 @default.
• W1978562493 hasConceptScore W1978562493C104317684 @default.
• W1978562493 hasConceptScore W1978562493C111337013 @default.
• W1978562493 hasConceptScore W1978562493C114246631 @default.
• W1978562493 hasConceptScore W1978562493C117262875 @default.
• W1978562493 hasConceptScore W1978562493C121332964 @default.
• W1978562493 hasConceptScore W1978562493C1491633281 @default.
• W1978562493 hasConceptScore W1978562493C153911025 @default.
• W1978562493 hasConceptScore W1978562493C181199279 @default.
• W1978562493 hasConceptScore W1978562493C18231593 @default.
• W1978562493 hasConceptScore W1978562493C185544564 @default.
• W1978562493 hasConceptScore W1978562493C185592680 @default.
• W1978562493 hasConceptScore W1978562493C2583947 @default.
• W1978562493 hasConceptScore W1978562493C2780340462 @default.
• W1978562493 hasConceptScore W1978562493C29537977 @default.
• W1978562493 hasConceptScore W1978562493C38240268 @default.
• W1978562493 hasConceptScore W1978562493C54355233 @default.
• W1978562493 hasConceptScore W1978562493C553184892 @default.
• W1978562493 hasConceptScore W1978562493C55493867 @default.
• W1978562493 hasConceptScore W1978562493C555283112 @default.
• W1978562493 hasConceptScore W1978562493C62112901 @default.
• W1978562493 hasConceptScore W1978562493C81885089 @default.
• W1978562493 hasConceptScore W1978562493C85813293 @default.
• W1978562493 hasConceptScore W1978562493C86803240 @default.
• W1978562493 hasConceptScore W1978562493C95444343 @default.
• W1978562493 hasIssue "2" @default.

• next