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- W1978583727 abstract "Abstract A new cuvet adapter designed for the Farrand A photoelectric fluorometer is found to be suitable for kinetic studies of enzymes. Added reactants are rapidly mixed with the substrate. The temperature is kept constant, and brief interposition of the standard is easily accomplished. The discharge of the fluorometer is automatically recorded. The curves after correcting for quenching are subjected to data computation and reproduced by a polynomial of the twelfth degree. The program includes derivation and calculations of Lineweaver-Burk diagrams. The experimental model is tested by studies on glucose phosphorylation by coupling to the pyruvate kinase/lactate dehydrogenase reactions. The consumption of NADH in the latter is followed by the decrease of the fluorescence. The ADP-ATP cycle operating in the system facilitates measurements at low ATP concentration since this can be maintained by appropriate amounts of enzymes. Further advantages of the system are the improved possibilities of measuring product inhibition from the phosphorylated compound and comparisons related to the same measuring product, when different compounds are phosphorylated." @default.
- W1978583727 created "2016-06-24" @default.
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- W1978583727 date "1968-02-01" @default.
- W1978583727 modified "2023-10-12" @default.
- W1978583727 title "A regenerating system for studies of phosphoryl transfer from ATP" @default.
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- W1978583727 doi "https://doi.org/10.1016/0003-2697(68)90308-4" @default.
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