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• W1978600754 endingPage "1022" @default.
• W1978600754 startingPage "1014" @default.
• W1978600754 abstract "1. Docosahexaenoic acid (DHA) and arachidonic acid (AA), polyunsaturated fatty acids (PUFAs), are important for central nervous system function during development and in various pathological states. Astrocytes are involved in the biosynthesis of PUFAs in neuronal tissue. Here, we investigated the mechanism of DHA and AA release in cultured rat brain astrocytes. 2. Primary astrocytes were cultured under standard conditions and prelabeled with [(14)C]DHA or with [(3)H]AA. Adenosine 5'-triphosphate (ATP) (20 micro M applied for 15 min), the P2Y receptor agonist, stimulates release of both DHA (289% of control) and AA (266% of control) from astrocytes. DHA release stimulated by ATP is mediated by Ca(2+)-independent phospholipase A(2) (iPLA(2)), since it is blocked by the selective iPLA(2) inhibitor 4-bromoenol lactone (BEL, 5 micro M) and is not affected either by removal of Ca(2+) from extracellular medium or by suppression of intracellular Ca(2+) release through PLC inhibitor (U73122, 5 micro M). 3. AA release, on the other hand, which is stimulated by ATP, is attributed to Ca(2+)-dependent cytosolic PLA(2) (cPLA(2)). AA release is abolished by U73122 and, by removal of extracellular Ca(2+), is insensitive to BEL and can be selectively suppressed by methyl arachidonyl fluorophosphonate (3 micro M), a general inhibitor of intracellular PLA(2) s. 4. Western blot analysis confirms the presence in rat brain astrocytes of 85 kDa cPLA(2) and 40 kDa protein reactive to iPLA(2) antibodies. 5. The influence of cAMP on regulation of PUFA release was investigated. Release of DHA is strongly amplified by the adenylyl cyclase activator forskolin (10 micro M), and by the protein kinase A (PKA) activator dibutyryl-cAMP (1 mM). In contrast, release of AA is not affected by forskolin or dibutyryl-cAMP, but is almost completely blocked by 2,3-dideoxyadenosine (20 micro M) and inhibited by 34% by H89 (10 micro M), inhibitors of adenylyl cyclase and PKA, respectively. 6. Other neuromediators, such as bradykinin, glutamate and thrombin, stimulate release of DHA and AA, which is comparable to the release stimulated by ATP. 7. Different sensitivities of iPLA(2) and cPLA(2) to Ca(2+) and cAMP reveal new pathways for the regulation of fatty acid release and reflect the significance of astrocytes in control of DHA and AA metabolism under normal and pathological conditions in brain." @default.
• W1978600754 created "2016-06-24" @default.
• W1978600754 creator A5012134134 @default.
• W1978600754 creator A5041657671 @default.
• W1978600754 creator A5046476020 @default.
• W1978600754 date "2003-07-01" @default.
• W1978600754 modified "2023-10-16" @default.
• W1978600754 title "Docosahexaenoic acid and arachidonic acid release in rat brain astrocytes is mediated by two separate isoforms of phospholipase A₂and is differently regulated by cyclic AMP and Ca²⁺" @default.
• W1978600754 cites W1259543534 @default.
• W1978600754 cites W1518541240 @default.
• W1978600754 cites W1540285469 @default.
• W1978600754 cites W1541909806 @default.
• W1978600754 cites W1664326306 @default.
• W1978600754 cites W1753591491 @default.
• W1978600754 cites W1772137817 @default.
• W1978600754 cites W1878752663 @default.
• W1978600754 cites W1882376829 @default.
• W1978600754 cites W1913518483 @default.
• W1978600754 cites W1928783644 @default.
• W1978600754 cites W1934742528 @default.
• W1978600754 cites W1975532690 @default.
• W1978600754 cites W1975608479 @default.
• W1978600754 cites W1977720833 @default.
• W1978600754 cites W1982135509 @default.
• W1978600754 cites W1990462078 @default.
• W1978600754 cites W1994497941 @default.
• W1978600754 cites W1995208754 @default.
• W1978600754 cites W1996024251 @default.
• W1978600754 cites W1998163989 @default.
• W1978600754 cites W2005966972 @default.
• W1978600754 cites W2010048722 @default.
• W1978600754 cites W2012220551 @default.
• W1978600754 cites W2012397093 @default.
• W1978600754 cites W2018084397 @default.
• W1978600754 cites W2025585810 @default.
• W1978600754 cites W2035532299 @default.
• W1978600754 cites W2041183642 @default.
• W1978600754 cites W2044535743 @default.
• W1978600754 cites W2048684437 @default.
• W1978600754 cites W2049192526 @default.
• W1978600754 cites W2052794702 @default.
• W1978600754 cites W2056327686 @default.
• W1978600754 cites W2062641944 @default.
• W1978600754 cites W2064117819 @default.
• W1978600754 cites W2066545353 @default.
• W1978600754 cites W2068990208 @default.
• W1978600754 cites W2069450664 @default.
• W1978600754 cites W2071936707 @default.
• W1978600754 cites W2075180304 @default.
• W1978600754 cites W2077394033 @default.
• W1978600754 cites W2080364836 @default.
• W1978600754 cites W2086654879 @default.
• W1978600754 cites W2093232668 @default.
• W1978600754 cites W2098006702 @default.
• W1978600754 cites W2122373956 @default.
• W1978600754 cites W2126276854 @default.
• W1978600754 cites W2131951611 @default.
• W1978600754 cites W2132305492 @default.
• W1978600754 cites W2133396109 @default.
• W1978600754 cites W2138721841 @default.
• W1978600754 cites W2141260882 @default.
• W1978600754 cites W2144450824 @default.
• W1978600754 cites W2146388445 @default.
• W1978600754 cites W2146408886 @default.
• W1978600754 cites W2149565581 @default.
• W1978600754 cites W2155511306 @default.
• W1978600754 cites W2166202725 @default.
• W1978600754 cites W2169044812 @default.
• W1978600754 cites W2182987870 @default.
• W1978600754 cites W2323797263 @default.
• W1978600754 cites W2324209228 @default.
• W1978600754 cites W2401267648 @default.
• W1978600754 cites W2411349429 @default.
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• W1978600754 doi "https://doi.org/10.1038/sj.bjp.0705326" @default.
• W1978600754 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1573920" @default.
• W1978600754 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12839876" @default.
• W1978600754 hasPublicationYear "2003" @default.
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