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- W1978633517 abstract "Ribosomal proteins from Mycobacterium tuberculosis H37 Rv were analyzed by Polyacrylamide gel electrophoresis and 73 proteins were separated. The 3OS subunit consists of 26 proteins, the 50S subunit of 41 proteins, 6 proteins of the ribosomes could not be shown in the subunits. The molecular weights for the proteins of the 30S subunit are 9100–43500, and for the proteins of the 50S subunit 8000–46000. The sedimentation coefficient have values of 28.8S and 47.7S. It was demonstrated that streptomycin-resistant cells in comparison to sensitive cells have two additional 30S proteins. Moreover, the protein L34 has changed its position. Kanamycin- and capreomycin-resistant cells have also two additional proteins, but viomycin shows no changes. Das Verteilungsmuster ribosomaler Protein von Mycobacterium tuberculosis H37 Rv wurde mit der Polyacrylamid-Gelelektrophorese dargestellt. 73 Proteine wurden identifiziert. 26 Proteine konnten der 3 OS-Untereinheit, 41 der 5 OS-Untereinheit zugeordnet werden. 6 Proteine der intakten Ribosomen wurden in den Untereinheiten nicht nachgewiesen. Für die Proteine der 30S-Untereinheit wurden Molekulargewichte von 9100 bis 43500 ermittelt, während bei den Proteinen der 50S-Untereinheit Molekulargewichte zwischen 8000 und 46000 bestimmt wurden. Für die Sedimentationskonstante ergaben sich Werte von 28,8S beziehungsweise 47,7S. Bei Streptomycin-resistenten Zellen traten im Vergleich zu den sensiblen 2 zusätzliche 30S-Proteine auf. Außerdem war das Protein L34 in seiner Lage verändert. Bei Kanamycin-und Capreomycin-Resistenz traten ebenfalls die beiden zusätzlichen 30S-Proteine auf. Das Proteinmuster Viomycin-resistenter Zellen war unverändert." @default.
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- W1978633517 date "1987-10-01" @default.
- W1978633517 modified "2023-09-24" @default.
- W1978633517 title "Comparison of ribosomes and ribosomal proteins of sensitive and resistant mycobacteria" @default.
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- W1978633517 doi "https://doi.org/10.1016/s0176-6724(87)80216-x" @default.
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