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• W1978636875 abstract "Summary Clathrin has been implicated in retromer-mediated trafficking, but its precise function remains elusive. Given the importance of retromers for efficient endosomal sorting, we have sought to clarify the relationship between clathrin and the SNX-BAR retromer. We find that the retromer SNX-BARs do not interact directly or indirectly with clathrin. In addition, we observe that SNX-BAR-retromer tubules and carriers are not clathrin coated. Furthermore, perturbing clathrin function, by overexpressing a dominant-negative clathrin or through suppression of clathrin expression, has no detectable effect on the frequency of SNX-BAR-retromer tubulation. We propose that SNX-BAR-retromer-mediated membrane deformation and carrier formation does not require clathrin, and hence the role of clathrin in SNX-BAR-retromer function would appear to lie in pre-SNX-BAR-retromer cargo sorting." @default.
• W1978636875 created "2016-06-24" @default.
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• W1978636875 date "2013-01-01" @default.
• W1978636875 modified "2023-09-27" @default.
• W1978636875 title "Clathrin is not required for SNX-BAR-retromer-mediated carrier formation" @default.
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• W1978636875 doi "https://doi.org/10.1242/jcs.112904" @default.
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