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- W1978651505 abstract "Single-stage surface replicas of treated or fresh pollen grains can be made ready for the electron microscope in 1.5 hr. The microspores are discharged into a drop of 50% acetone on a 1 cm square of cleaved mica and air dried. Carbon is evaporated to a film thickness of 35 mμ during rotation of the mica support. The carbon film and microspores are parted from the mica with water and heated in 2-aminoethanol at 145-155 C for 10 min to 3 hr. The replicas are then washed 5 min or longer on water at 90 C and picked up on electron microscope grids. The resulting self-shadowed surface replica can be immediately observed by electron microscopy." @default.
- W1978651505 created "2016-06-24" @default.
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- W1978651505 date "1966-01-01" @default.
- W1978651505 modified "2023-10-16" @default.
- W1978651505 title "Single-Stage Carbon Replicas of Microspores" @default.
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- W1978651505 doi "https://doi.org/10.3109/10520296609116324" @default.
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