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- W1978652803 abstract "Is there a proven alternative to the basic semen analysis (SA)? HA and CAP are the true measure of fertilizing function. Shouldn't we resolve two nagging questions? 1) Any CAP/ HA test must be wedded to pregnancy. 2) How do CASA definitions for sperm HA compare with one another? IVOS instruments and deep chambers were used. HA studies compared the 3 most cited HA systems: Burkman 1990, Mortimer and Mortimer 1990, and also Mortimer & Mortimer (M&M, 1995). The IVOS Sort program defined HA swimming patterns: thrash, star, helical, & circling, or all four together. HDATA pulled data from each analysis into Excel files for later HA sorting according to 12 equations. Over 50,000 sperm were evaluated, from 9 different donors. Sperm were sampled at all stages of CAP. 1) % of sperm with the individual HA patterns, or ALL HA, when Burkman HA criteria were put into SORT; 2) and when the sperm were reassessed using M&M 1990 criteria; 3) The same comparison was performed, but using the 1995 criteria; 4) Modifications to the 1995 criteria were tested, especially amplitude. Statistics: regression, ANOVA, chi-squared. Readings of human sperm using the Burkman 1990 criteria were compared to the M&M 1990 definitions: no statistical difference (P equal to 0.47). Comparison of Burkman criteria for HA to M&M 1995 values gave a P value less than 0.001. Parallel comparisons were made for individual patterns. Burkman values for Helical, Thrash and Star, added together, were virtually equal to the 1995 result. Inclusion of Circling dropped dramatically with M&M 1995. Pregnancy, as an endpoint, is our gold standard (see other abstract). We find no Pregnancy data as validation for the 1995 paper. LifeCell Dx can provide guidelines for initiating Clear CASA in any lab. All labs must validate their own specific protocols for Clear CASA (medium, temperature, sperm handling, etc.). We emphasize that Circling HA adheres o the classical drawings of HA." @default.
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- W1978652803 date "2013-09-01" @default.
- W1978652803 modified "2023-09-26" @default.
- W1978652803 title "Defining the fertile man with clear CASA: capacitation (CAP) and hyperactivation (HA)" @default.
- W1978652803 doi "https://doi.org/10.1016/j.fertnstert.2013.07.541" @default.
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