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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978654705> ?p ?o ?g. }

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• W1978654705 abstract "DNA-sequences coding for two DesPro(2) aprotinin variants were expressed in the methylotrophic yeast Hansenula polymorpha from a strong inducible promoter element derived from the MOX gene, a key gene of methanol metabolism. For secretion the coding sequences were fused to the KEX2 recognition site of the S. cerevisiae-derived MFα1 preproleader sequence. Correct processing of the precursor molecules and efficient secretion of the mature proteins was observed. A pH/pO2-controlled C-source feeding mode was applied for fermentations on a 10 litre scale. In cultures of a transformant strain harbouring 20 copies of the aprotinin expression cassette a yield of 350 mg/litre could be obtained. The secreted recombinant products were purified in a simple two-step isolation procedure employing an expanded bed adsorption as an initial purification step." @default.
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• W1978654705 date "1996-09-01" @default.
• W1978654705 modified "2023-09-27" @default.
• W1978654705 title "Production of two aprotinin variants in Hansenula polymorpha" @default.
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• W1978654705 doi "https://doi.org/10.1016/s0032-9592(96)00018-0" @default.
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