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- W1978665764 abstract "A 5-day-old adult stage-specific cDNA fragment from Trichinella spiralis was identified by suppression subtractive hybridization and was used as a probe to screen the cDNA library. The cDNA sequence coding for a putative T. spiralis cuticle collagen was isolated. The cDNA encoded an open reading frame of 343 amino acid residues with molecular weight of 35.1 kDa. The deduced protein contained an N-terminal signal peptide, a nematode cuticle collagen N-terminal domain and a collagen triple helix repeat domain. Searches in GenBank using BLASTP showed up to 47% identity to cuticle collagens from other nematodes. Southern blot analysis of genomic DNA indicated this gene was present as a single copy in T. spiralis genome." @default.
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- W1978665764 date "2005-09-01" @default.
- W1978665764 modified "2023-09-30" @default.
- W1978665764 title "Molecular cloning of a cDNA encoding a putative cuticle collagen of Trichinella spiralis" @default.
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- W1978665764 doi "https://doi.org/10.1016/j.vetpar.2005.05.020" @default.
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