Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978667085> ?p ?o ?g. }
- W1978667085 endingPage "2462" @default.
- W1978667085 startingPage "2454" @default.
- W1978667085 abstract "Obesity is associated with insulin resistance and type II diabetes mellitus. In the present study, we have characterized hepatic insulin receptor function in two animal models of obesity: the Zucker fatty rat (ZFR), a model of genetic obesity with severe hyperinsulinemia, and the Sprague-Dawley rat with dietary obesity, a model of acquired obesity. Zucker fatty rats were also treated with streptozotocin (STZ) in an effort to examine the effects of relative insulin deficiency and hyperglycemia in the setting of obesity. Using wheat germ agglutinin-purified insulin receptor extracted from liver, no significant difference in insulin binding was identified in either model of obesity. β-Subunit autophosphorylation was significantly decreased in both obese models relative to that in controls (72% in the obese ZFR and 49% in the overfed Sprague-Dawley model). Kinase activity, as measured by phosphorylation of the 1142–1153 synthetic peptide, was also decreased in both models of obesity by 22% and 64%, respectively. In the Zucker rat, STZ treatment led to an 80% increase in receptor concentration and a further 70% increase in β-subunit autophosphorylation per receptor, whereas tyrosine kinase activity toward substrate was not altered. Since kinase activity is closely linked to autophosphorylation, we determined the fraction of autophosphorylated (activated) receptors vs. nonphosphorylated (inactive) receptors by using antiphosphotyrosine antibody to precipitate receptors bound with [125I]insulin. There was no significant difference in the percentage of activated insulin receptors in the dietary obese, ZFR, or STZ-treated Zucker rat vs. that in the controls. In all models, the percentage of activated receptors ranged from 32-46% of the total receptor pool. These data suggest that in genetic and acquired obesity, autophosphorylation of the β-subunit is reduced and is a limiting factor in insulin receptor activation. A similar fraction of all receptors appears to undergo some level of autophosphorylation; however, full autophosphorylation and, thus, activation of the receptor do not occur, and this results in a decrease in kinase activity. This block in autophosphorylation may account for significant reductions in insulin receptor kinase function in obesity." @default.
- W1978667085 created "2016-06-24" @default.
- W1978667085 creator A5020304503 @default.
- W1978667085 creator A5047308402 @default.
- W1978667085 creator A5051737440 @default.
- W1978667085 date "1989-11-01" @default.
- W1978667085 modified "2023-10-16" @default.
- W1978667085 title "Alterations in the Hepatic Insulin Receptor Kinase in Genetic and Acquired Obesity in Rats*" @default.
- W1978667085 cites W115701967 @default.
- W1978667085 cites W147537042 @default.
- W1978667085 cites W1481444615 @default.
- W1978667085 cites W1572696798 @default.
- W1978667085 cites W1608611896 @default.
- W1978667085 cites W1624505911 @default.
- W1978667085 cites W1668568781 @default.
- W1978667085 cites W189341708 @default.
- W1978667085 cites W1900727795 @default.
- W1978667085 cites W1976667813 @default.
- W1978667085 cites W1986930204 @default.
- W1978667085 cites W1994807734 @default.
- W1978667085 cites W1996125730 @default.
- W1978667085 cites W2005959762 @default.
- W1978667085 cites W2013648465 @default.
- W1978667085 cites W2014496040 @default.
- W1978667085 cites W2041827832 @default.
- W1978667085 cites W2046802065 @default.
- W1978667085 cites W2048793124 @default.
- W1978667085 cites W2054575524 @default.
- W1978667085 cites W2062299625 @default.
- W1978667085 cites W2072251035 @default.
- W1978667085 cites W2088067419 @default.
- W1978667085 cites W2092767995 @default.
- W1978667085 cites W2100837269 @default.
- W1978667085 cites W2104713078 @default.
- W1978667085 cites W2115093786 @default.
- W1978667085 cites W2128635872 @default.
- W1978667085 cites W2151299241 @default.
- W1978667085 cites W2164884154 @default.
- W1978667085 cites W2343157373 @default.
- W1978667085 cites W2394646614 @default.
- W1978667085 cites W2434039693 @default.
- W1978667085 cites W28452575 @default.
- W1978667085 cites W372838896 @default.
- W1978667085 cites W44668478 @default.
- W1978667085 doi "https://doi.org/10.1210/endo-125-5-2454" @default.
- W1978667085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2551653" @default.
- W1978667085 hasPublicationYear "1989" @default.
- W1978667085 type Work @default.
- W1978667085 sameAs 1978667085 @default.
- W1978667085 citedByCount "25" @default.
- W1978667085 countsByYear W19786670852013 @default.
- W1978667085 countsByYear W19786670852015 @default.
- W1978667085 countsByYear W19786670852022 @default.
- W1978667085 crossrefType "journal-article" @default.
- W1978667085 hasAuthorship W1978667085A5020304503 @default.
- W1978667085 hasAuthorship W1978667085A5047308402 @default.
- W1978667085 hasAuthorship W1978667085A5051737440 @default.
- W1978667085 hasConcept C112446052 @default.
- W1978667085 hasConcept C126322002 @default.
- W1978667085 hasConcept C134018914 @default.
- W1978667085 hasConcept C150109051 @default.
- W1978667085 hasConcept C170493617 @default.
- W1978667085 hasConcept C184235292 @default.
- W1978667085 hasConcept C197902417 @default.
- W1978667085 hasConcept C2777391703 @default.
- W1978667085 hasConcept C2779306644 @default.
- W1978667085 hasConcept C2780988686 @default.
- W1978667085 hasConcept C55493867 @default.
- W1978667085 hasConcept C71924100 @default.
- W1978667085 hasConcept C86803240 @default.
- W1978667085 hasConcept C97029542 @default.
- W1978667085 hasConceptScore W1978667085C112446052 @default.
- W1978667085 hasConceptScore W1978667085C126322002 @default.
- W1978667085 hasConceptScore W1978667085C134018914 @default.
- W1978667085 hasConceptScore W1978667085C150109051 @default.
- W1978667085 hasConceptScore W1978667085C170493617 @default.
- W1978667085 hasConceptScore W1978667085C184235292 @default.
- W1978667085 hasConceptScore W1978667085C197902417 @default.
- W1978667085 hasConceptScore W1978667085C2777391703 @default.
- W1978667085 hasConceptScore W1978667085C2779306644 @default.
- W1978667085 hasConceptScore W1978667085C2780988686 @default.
- W1978667085 hasConceptScore W1978667085C55493867 @default.
- W1978667085 hasConceptScore W1978667085C71924100 @default.
- W1978667085 hasConceptScore W1978667085C86803240 @default.
- W1978667085 hasConceptScore W1978667085C97029542 @default.
- W1978667085 hasIssue "5" @default.
- W1978667085 hasLocation W19786670851 @default.
- W1978667085 hasLocation W19786670852 @default.
- W1978667085 hasOpenAccess W1978667085 @default.
- W1978667085 hasPrimaryLocation W19786670851 @default.
- W1978667085 hasRelatedWork W1978667085 @default.
- W1978667085 hasRelatedWork W1991367927 @default.
- W1978667085 hasRelatedWork W2001093010 @default.
- W1978667085 hasRelatedWork W2028242557 @default.
- W1978667085 hasRelatedWork W2151350502 @default.
- W1978667085 hasRelatedWork W2155981800 @default.
- W1978667085 hasRelatedWork W2197098620 @default.
- W1978667085 hasRelatedWork W3084048874 @default.