FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978668090> ?p ?o ?g. }

• W1978668090 endingPage "206" @default.
• W1978668090 startingPage "193" @default.
• W1978668090 abstract "In order to explore the use of exchangeable poly(ethylene glycol) (PEG)-modified diacylphosphatidylethanolamines (PE) to temporarily shield binding ligands attached to the surface of liposomes, a model reaction based on inhibition and subsequent recovery of biotinylated liposome binding to streptavidin immobilized on superparamagnetic iron oxide particles (SA magnetic particles) was developed. PEG-lipid incorporation into biotinylated liposomes decreased liposome binding to SA magnetic particles in a non-linear fashion, where as little as 0.1 mol% PEG-PE resulted in a 20% decrease in binding. Using an assay based on inhibition of binding, PEG 2000 -PE transfer from donor liposomes to biotinylated acceptor liposomes could be measured. The influence of temperature and acyl chain composition on the transfer of PEG-diacyl PEs from donor liposomes to acceptor liposomes, consisting of 1,2-dioleoyl- sn -glycero-3-phosphocholine, cholesterol and N -((6-biotinoyl)amino)hexanoyl)-1,2-distearoyl- sn -glycero-3-phosphoethanolamine (54.9:45:0.1 mole ratio), was measured. Donor liposomes were prepared using 1,2-distearoyl- sn -glycero-3-phosphocholine (50 mol%), cholesterol (45 mol%) and 5 mol% of either PEG-derivatized 1,2-dimyristoyl- sn -glycero-3-phosphoethanolamine (DMPE-PEG 2000 ), 1,2-dipalmitoyl- sn -glycero-3-phosphoethanolamine (DPPE-PEG 2000 ), or 1,2-distearoyl- sn -glycero-3-phosphoethanolamine (DSPE-PEG 2000 ). Transfer of DSPE-PEG 2000 to the donor liposomes was not detected under the conditions employed. In contrast, DMPE-PEG 2000 was transferred efficiently even at 4°C. Using an acceptor to donor liposome ratio of 1:4, the time required for DMPE-PEG 2000 to become evenly distributed between the two liposome populations ( T EQ ) at 4°C and 37°C was approx. 2 and <0.5 h, respectively. An increase in acyl chain length from C14:0 to C16:0 of the PEG-lipid resulted in a significant reduction in the rate of transfer as measured by this assay. The transfer of PEG-lipid out of biotinylated liposomes was also studied in mice following intravenous administration. The relative rates of transfer for the various PEG-lipids were found to be comparable under in vivo and in vitro conditions. These results suggest that it is possible to design targeted liposomes with the targeting ligand protected while in the circulation through the use of PEG-lipids that are selected on the basis of exchange characteristics which result in exposure of the shielded ligand following localization within a target tissue." @default.
• W1978668090 created "2016-06-24" @default.
• W1978668090 creator A5005293833 @default.
• W1978668090 creator A5020081255 @default.
• W1978668090 creator A5070792731 @default.
• W1978668090 creator A5074722318 @default.
• W1978668090 date "2001-08-01" @default.
• W1978668090 modified "2023-09-29" @default.
• W1978668090 title "Intermembrane transfer of polyethylene glycol-modified phosphatidylethanolamine as a means to reveal surface-associated binding ligands on liposomes" @default.
• W1978668090 cites W1511099869 @default.
• W1978668090 cites W1511856286 @default.
• W1978668090 cites W1966824444 @default.
• W1978668090 cites W1970958568 @default.
• W1978668090 cites W1981057166 @default.
• W1978668090 cites W1983671192 @default.
• W1978668090 cites W1985342306 @default.
• W1978668090 cites W1986516374 @default.
• W1978668090 cites W1993342139 @default.
• W1978668090 cites W1994738464 @default.
• W1978668090 cites W1996695748 @default.
• W1978668090 cites W2000179170 @default.
• W1978668090 cites W2005556449 @default.
• W1978668090 cites W2008594853 @default.
• W1978668090 cites W2009199932 @default.
• W1978668090 cites W2015839115 @default.
• W1978668090 cites W2022384016 @default.
• W1978668090 cites W2031446684 @default.
• W1978668090 cites W2032369546 @default.
• W1978668090 cites W2048764833 @default.
• W1978668090 cites W2053171199 @default.
• W1978668090 cites W2061672022 @default.
• W1978668090 cites W2065805539 @default.
• W1978668090 cites W2068354981 @default.
• W1978668090 cites W2079711588 @default.
• W1978668090 cites W2083463630 @default.
• W1978668090 cites W2089204232 @default.
• W1978668090 cites W2093007266 @default.
• W1978668090 cites W2094261110 @default.
• W1978668090 cites W2095385626 @default.
• W1978668090 cites W2101918969 @default.
• W1978668090 cites W2107919521 @default.
• W1978668090 cites W2110630416 @default.
• W1978668090 cites W2131653836 @default.
• W1978668090 cites W2137000696 @default.
• W1978668090 cites W2065245211 @default.
• W1978668090 doi "https://doi.org/10.1016/s0005-2736(01)00351-0" @default.
• W1978668090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11470091" @default.
• W1978668090 hasPublicationYear "2001" @default.
• W1978668090 type Work @default.
• W1978668090 sameAs 1978668090 @default.
• W1978668090 citedByCount "35" @default.
• W1978668090 countsByYear W19786680902012 @default.
• W1978668090 countsByYear W19786680902013 @default.
• W1978668090 countsByYear W19786680902016 @default.
• W1978668090 countsByYear W19786680902017 @default.
• W1978668090 countsByYear W19786680902018 @default.
• W1978668090 countsByYear W19786680902019 @default.
• W1978668090 countsByYear W19786680902020 @default.
• W1978668090 countsByYear W19786680902021 @default.
• W1978668090 countsByYear W19786680902022 @default.
• W1978668090 countsByYear W19786680902023 @default.
• W1978668090 crossrefType "journal-article" @default.
• W1978668090 hasAuthorship W1978668090A5005293833 @default.
• W1978668090 hasAuthorship W1978668090A5020081255 @default.
• W1978668090 hasAuthorship W1978668090A5070792731 @default.
• W1978668090 hasAuthorship W1978668090A5074722318 @default.
• W1978668090 hasBestOaLocation W19786680901 @default.
• W1978668090 hasConcept C10138342 @default.
• W1978668090 hasConcept C13965031 @default.
• W1978668090 hasConcept C162324750 @default.
• W1978668090 hasConcept C178790620 @default.
• W1978668090 hasConcept C185154212 @default.
• W1978668090 hasConcept C185592680 @default.
• W1978668090 hasConcept C204628709 @default.
• W1978668090 hasConcept C2776330855 @default.
• W1978668090 hasConcept C2776964284 @default.
• W1978668090 hasConcept C2777516009 @default.
• W1978668090 hasConcept C2778918659 @default.
• W1978668090 hasConcept C2781170229 @default.
• W1978668090 hasConcept C41625074 @default.
• W1978668090 hasConcept C43617362 @default.
• W1978668090 hasConcept C54400483 @default.
• W1978668090 hasConcept C55493867 @default.
• W1978668090 hasConceptScore W1978668090C10138342 @default.
• W1978668090 hasConceptScore W1978668090C13965031 @default.
• W1978668090 hasConceptScore W1978668090C162324750 @default.
• W1978668090 hasConceptScore W1978668090C178790620 @default.
• W1978668090 hasConceptScore W1978668090C185154212 @default.
• W1978668090 hasConceptScore W1978668090C185592680 @default.
• W1978668090 hasConceptScore W1978668090C204628709 @default.
• W1978668090 hasConceptScore W1978668090C2776330855 @default.
• W1978668090 hasConceptScore W1978668090C2776964284 @default.
• W1978668090 hasConceptScore W1978668090C2777516009 @default.
• W1978668090 hasConceptScore W1978668090C2778918659 @default.
• W1978668090 hasConceptScore W1978668090C2781170229 @default.
• W1978668090 hasConceptScore W1978668090C41625074 @default.
• W1978668090 hasConceptScore W1978668090C43617362 @default.
• W1978668090 hasConceptScore W1978668090C54400483 @default.

• next