Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978690746> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1978690746 abstract "Background: The development of cell therapy for the rescue of damaged heart muscle is a major area of inquiry. Within this context, the establishment of a cardiogenic cell line may remarkably facilitate the molecular dissection of cardiac fate specification, a low-efficiency and still poorly understood process, paving the way for novel approaches in the use of stem cells for cardiac repair. Methods & results: We used GTR1 cells, a derivative of mouse R1 embryonic stem cells bearing the puromycin-resistance gene driven by the cardiomyocyte-specific α-myosin heavy chain promoter, affording a gene trapping selection of a virtually pure population of embryonic stem cell-derived cardiomyocytes. Third-generation lentiviral vectors were used to overexpress the prodynorphin gene, previously shown to orchestrate a dynorphinergic system acting as a major conductor of embryonic stem cell cardiogenesis. Lentiviral prodynorphin transduction remarkably enhanced the transcription of GATA-4 and Nkx-2.5, two cardiac lineage-promoting genes, resulting in a dramatic increase in the number of spontaneously beating cardiomyocytes. Transduced cells also exhibited a subcellular redistribution patterning of protein kinase C-β, -δ and -ε, a major requirement in cardiac lineage commitment. This activation resulted from a sustained increase in the transcription of targeted protein kinase C genes. Prodynorphin transduction was selective in nature and failed to activate genes responsible for skeletal myogenesis or neuronal specification. Conclusions: The cell line developed in this study provides a powerful in vitro model of cardiomyogenesis that may help clarify the cascade of transcriptional activation and signaling networks that push multipotent cells to take on the identity of a cardiac myocyte." @default.
- W1978690746 created "2016-06-24" @default.
- W1978690746 creator A5002819777 @default.
- W1978690746 creator A5012812911 @default.
- W1978690746 creator A5013202145 @default.
- W1978690746 creator A5024619548 @default.
- W1978690746 creator A5051416401 @default.
- W1978690746 creator A5084682964 @default.
- W1978690746 creator A5085217099 @default.
- W1978690746 creator A5086181630 @default.
- W1978690746 date "2007-03-01" @default.
- W1978690746 modified "2023-09-27" @default.
- W1978690746 title "Creating prodynorphin-expressing stem cells alerted for a high-throughput of cardiogenic commitment" @default.
- W1978690746 cites W1631250427 @default.
- W1978690746 cites W1865658459 @default.
- W1978690746 cites W1960160851 @default.
- W1978690746 cites W1966387132 @default.
- W1978690746 cites W1977437881 @default.
- W1978690746 cites W1979103623 @default.
- W1978690746 cites W1994006339 @default.
- W1978690746 cites W2013153413 @default.
- W1978690746 cites W2028036560 @default.
- W1978690746 cites W2040686597 @default.
- W1978690746 cites W2073191701 @default.
- W1978690746 cites W2073403442 @default.
- W1978690746 cites W2075344844 @default.
- W1978690746 cites W2075563634 @default.
- W1978690746 cites W2080163359 @default.
- W1978690746 cites W2102609766 @default.
- W1978690746 cites W2115579253 @default.
- W1978690746 cites W2125155374 @default.
- W1978690746 cites W2129957319 @default.
- W1978690746 cites W2148220540 @default.
- W1978690746 cites W2152157399 @default.
- W1978690746 cites W2156227047 @default.
- W1978690746 cites W2164506013 @default.
- W1978690746 cites W2164855942 @default.
- W1978690746 cites W2165816043 @default.
- W1978690746 cites W2167523976 @default.
- W1978690746 doi "https://doi.org/10.2217/17460751.2.2.193" @default.
- W1978690746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17465751" @default.
- W1978690746 hasPublicationYear "2007" @default.
- W1978690746 type Work @default.
- W1978690746 sameAs 1978690746 @default.
- W1978690746 citedByCount "8" @default.
- W1978690746 countsByYear W19786907462012 @default.
- W1978690746 countsByYear W19786907462013 @default.
- W1978690746 countsByYear W19786907462019 @default.
- W1978690746 countsByYear W19786907462022 @default.
- W1978690746 crossrefType "journal-article" @default.
- W1978690746 hasAuthorship W1978690746A5002819777 @default.
- W1978690746 hasAuthorship W1978690746A5012812911 @default.
- W1978690746 hasAuthorship W1978690746A5013202145 @default.
- W1978690746 hasAuthorship W1978690746A5024619548 @default.
- W1978690746 hasAuthorship W1978690746A5051416401 @default.
- W1978690746 hasAuthorship W1978690746A5084682964 @default.
- W1978690746 hasAuthorship W1978690746A5085217099 @default.
- W1978690746 hasAuthorship W1978690746A5086181630 @default.
- W1978690746 hasConcept C104317684 @default.
- W1978690746 hasConcept C145103041 @default.
- W1978690746 hasConcept C151730666 @default.
- W1978690746 hasConcept C2779343474 @default.
- W1978690746 hasConcept C28328180 @default.
- W1978690746 hasConcept C2908647359 @default.
- W1978690746 hasConcept C54355233 @default.
- W1978690746 hasConcept C71924100 @default.
- W1978690746 hasConcept C86803240 @default.
- W1978690746 hasConcept C95444343 @default.
- W1978690746 hasConcept C99454951 @default.
- W1978690746 hasConceptScore W1978690746C104317684 @default.
- W1978690746 hasConceptScore W1978690746C145103041 @default.
- W1978690746 hasConceptScore W1978690746C151730666 @default.
- W1978690746 hasConceptScore W1978690746C2779343474 @default.
- W1978690746 hasConceptScore W1978690746C28328180 @default.
- W1978690746 hasConceptScore W1978690746C2908647359 @default.
- W1978690746 hasConceptScore W1978690746C54355233 @default.
- W1978690746 hasConceptScore W1978690746C71924100 @default.
- W1978690746 hasConceptScore W1978690746C86803240 @default.
- W1978690746 hasConceptScore W1978690746C95444343 @default.
- W1978690746 hasConceptScore W1978690746C99454951 @default.
- W1978690746 hasLocation W19786907461 @default.
- W1978690746 hasLocation W19786907462 @default.
- W1978690746 hasOpenAccess W1978690746 @default.
- W1978690746 hasPrimaryLocation W19786907461 @default.
- W1978690746 hasRelatedWork W1978835772 @default.
- W1978690746 hasRelatedWork W2009519731 @default.
- W1978690746 hasRelatedWork W2038174987 @default.
- W1978690746 hasRelatedWork W2055156816 @default.
- W1978690746 hasRelatedWork W2106115330 @default.
- W1978690746 hasRelatedWork W2138796069 @default.
- W1978690746 hasRelatedWork W2207036327 @default.
- W1978690746 hasRelatedWork W2407967826 @default.
- W1978690746 hasRelatedWork W2416444910 @default.
- W1978690746 hasRelatedWork W4238775786 @default.
- W1978690746 isParatext "false" @default.
- W1978690746 isRetracted "false" @default.
- W1978690746 magId "1978690746" @default.
- W1978690746 workType "article" @default.