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- W1978691172 abstract "A laser‐light pulse counting method was developed and investigated for its ability automatically to count acridine orange‐stained bacteria and microcolonies of bacteria on the surface of polycarbonate membrane filters. The system consisted of an Argon laser which delivered a 5 μm diameter spot of coherent 488 nm wavelength light which was raster scanned across a 5×2 mm area of the membrane. Fluorescence pulses of orange light (ca 650 nm) were detected via a dichroic mirror and barrier filter with a photomultiplier tube. For microcolony preparations a good relationship between the number of light pulses and cell density down to below 102/ml was observed, but at lower cell densities counting was not reliable, probably because of fluorescent debris. The method was also able to count single cells, but background auto‐fluorescence and photo‐bleaching produced a high and variable background signal in some samples." @default.
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- W1978691172 date "1989-02-01" @default.
- W1978691172 modified "2023-09-27" @default.
- W1978691172 title "A laser-light pulse counting method for automatic and sensitive counting of bacteria stained with acridine orange" @default.
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- W1978691172 doi "https://doi.org/10.1111/j.1365-2672.1989.tb02465.x" @default.
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