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• W1978736039 abstract "Detection of disseminated tumor cells in the bone marrow may provide important prognostic information in breast cancer patients. With few exceptions the number of stained cells scored as cancer is very low; there may be only 1 cell per slide. This makes definitive interpretation of cancer in marrow challenging. False-positive staining of marrow cells with cytokeratin (CK) antibody is relatively common and makes interpretation more difficult. In this report we focus on false-positive staining of marrow specimens from breast cancer patients and noncancer controls and demonstrate that the frequency of false-positive events is common. Bone marrow was collected from 23 cancer-free donors and 60 breast cancer patients. Samples were processed by Ficoll density gradient centrifugation and slides were prepared for immunocytochemical staining with CK and irrelevant (IR) antibody. Slides were evaluated manually and positive cells were categorized as tumor cells, hematopoetic cells, or questionable cells. False-positive staining events were commonly observed in noncancer cases stained with CK or IR antibodies and in breast cancer cases stained with IR antibody. There was little difference in the number of breast cancer marrow specimens scored as tumor cells regardless of whether the antibody used was CK or IR. It is important to devise improved criteria and methods for accurate detection and interpretation of disseminated tumor cells in the marrow of breast cancer patients." @default.
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• W1978736039 date "2009-10-01" @default.
• W1978736039 modified "2023-10-18" @default.
• W1978736039 title "Cytokeratin-positive Cells in the Bone Marrow of Breast Cancer Patients and Noncancer Donors" @default.
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• W1978736039 doi "https://doi.org/10.1097/pai.0b013e31819f4d6b" @default.
• W1978736039 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2836413" @default.
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