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• W1978794695 abstract "Overlapping cDNAs encoding dihydropyrimidine dehydrogenase (DPD), the rate-limiting enzyme of pyrimidine degradation, were isolated from a rat liver cDNA library, and a 4, 353 by cDNA sequence with a 3, 075 by open reading frame encoding a polypeptide of 1, 025 residues with a molecular mass of 111, 458 Da was determined. Western blot analysis of COS-7 cells that were transfected with an RT-PCR-derived DPD cDNA showed that the product from the cDNA binds to an antiserum for DPD. Northern blot analysis was performed by using a partial fragment of the cloned cDNA as a probe. The mRNA level, the protein level, and the activity of DPD in the rat liver increased to the adult level by 3 mo of age. The levels of DPD in the liver of adrenalectomized rats increased 48 h after glucocorticoid administration. The increase of DPD after birth may be caused by the increase in glucocorticoid level." @default.
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• W1978794695 date "1998-01-01" @default.
• W1978794695 modified "2023-10-05" @default.
• W1978794695 title "Cloning of Rat Dihydropyrimidine Dehydrogenase and Correlation of Its mRNA Increase in the Rat Liver with Age." @default.
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• W1978794695 doi "https://doi.org/10.3177/jnsv.44.537" @default.
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