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- W1978797788 abstract "Ribosomal RNA promoters of Escherichia coli are probably the strongest promoters in vivo and they can be used on plasmid vectors to express protein-coding sequences at a high rate. In fact, the P2 promoter of the rrnB gene is stronger (in vivo) than the tac promoter, which has a perfect consensus sequence. Conversion of the rrnB P2 promoter sequence to consensus significantly increases in vivo promoter strength. The removal of four nucleotides downstream of the -10 region also increases the strength of this promoter. On the other hand, shifting of the A + T-rich region upstream of this promoter by an 11-bp insertion drastically decreases in vivo activity. It is concluded that the two functionally important hexanucleotide sequences, -35 and -10, are necessary but not sufficient factors for the optimalization of in vivo promoter strength." @default.
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- W1978797788 date "1989-05-01" @default.
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- W1978797788 title "The structural basis of the high in vivo strength of the rRNA P2 promoter of Escherichia coli" @default.
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- W1978797788 doi "https://doi.org/10.1016/0378-1119(89)90328-4" @default.
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