FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978803247> ?p ?o ?g. }

• W1978803247 endingPage "2008" @default.
• W1978803247 startingPage "2003" @default.
• W1978803247 abstract "Human papilloma virus (HPV) type 16 has an established association with anogenital carcinoma, and to some extent with human oral squamous cell carcinoma. We hypothesize that HPV type 16 is capable of inducing chromosomal and cell cycle changes in cultured oral epithelial cells. Normal human oral epithelial cells were immortalized with recom-binant retrovirus containing the E6/E7 open reading frames of HPV type 16. These cells have been in culture for more than 350 passages and over 4 years. Flow cytometry demonstrated an average of 42% nuclear aneuploidy in HPV 16-immortalized cells; 16% in normal controls (probably tetrasomy). Cytogenetic analysis demonstrated significant progression of chromosomal abnormalities. Cells at early passage (p10) showed trisomy 20, with no other major changes. At passage 18, trisomy lq and monosomy 13 were seen in addition to trisomy 20. At passage 61 there were two distinct cell populations (‘a’ and ‘b’), with multiple chromosomal changes including trisomy 5q,14,20 in one line and 7p,9q,11q in the other. Both populations had monosomy 3p, with monosomy 8p in one population and monosomy 13 in the other. At passage 136, the cells were essentially identical to population ‘b’ of passage 61. At this passage, mutation of the p53 gene was detected at codon 273 of exon 8, with G to T conversion (Arg to Leu). This was absent in the normal cells from which this line was developed. Passage 262 contained the two major cell populations, each with a sub-group with additional chromosomal changes such as l0p monosomy. Cells from passages 217 and 305 were injected into nude mice a year apart Both failed to produce tumors, as did normal cells. In conclusion, we present an HPV type 16-immortalized oral epithelial cell line (IHGK) with extensive and progressive chromosomal abnormalities, invasive growth in culture and yet no tumor formation in nude mice. We suggest that the question as to whether HPV alone can induce transformation is still open." @default.
• W1978803247 created "2016-06-24" @default.
• W1978803247 creator A5019253075 @default.
• W1978803247 creator A5062194105 @default.
• W1978803247 creator A5063367973 @default.
• W1978803247 creator A5067240879 @default.
• W1978803247 date "1996-09-01" @default.
• W1978803247 modified "2023-09-23" @default.
• W1978803247 title "Chromosomal abnormalities in HPV-16-immortalized oral epithelial cells" @default.
• W1978803247 cites W1500130815 @default.
• W1978803247 cites W1510715774 @default.
• W1978803247 cites W152013658 @default.
• W1978803247 cites W1522666032 @default.
• W1978803247 cites W1602188512 @default.
• W1978803247 cites W1722478811 @default.
• W1978803247 cites W1767781328 @default.
• W1978803247 cites W1858263251 @default.
• W1978803247 cites W1907535383 @default.
• W1978803247 cites W1926572842 @default.
• W1978803247 cites W1942260657 @default.
• W1978803247 cites W1967492371 @default.
• W1978803247 cites W1967960141 @default.
• W1978803247 cites W1969074708 @default.
• W1978803247 cites W1976051908 @default.
• W1978803247 cites W1976202208 @default.
• W1978803247 cites W1990010614 @default.
• W1978803247 cites W1992476328 @default.
• W1978803247 cites W1999053674 @default.
• W1978803247 cites W2018733832 @default.
• W1978803247 cites W2020415912 @default.
• W1978803247 cites W2029590593 @default.
• W1978803247 cites W2033882604 @default.
• W1978803247 cites W2038500217 @default.
• W1978803247 cites W2039382131 @default.
• W1978803247 cites W2045154524 @default.
• W1978803247 cites W2052144512 @default.
• W1978803247 cites W2052303436 @default.
• W1978803247 cites W2057501779 @default.
• W1978803247 cites W2060786724 @default.
• W1978803247 cites W2070642970 @default.
• W1978803247 cites W2074959117 @default.
• W1978803247 cites W2087811918 @default.
• W1978803247 cites W2120747276 @default.
• W1978803247 cites W2132895978 @default.
• W1978803247 cites W2138034395 @default.
• W1978803247 cites W2143080883 @default.
• W1978803247 cites W2150097377 @default.
• W1978803247 cites W2151066134 @default.
• W1978803247 cites W2153909900 @default.
• W1978803247 cites W2156872475 @default.
• W1978803247 cites W2200171971 @default.
• W1978803247 cites W2397278930 @default.
• W1978803247 cites W2477177595 @default.
• W1978803247 cites W2503114490 @default.
• W1978803247 cites W27510651 @default.
• W1978803247 cites W2281885676 @default.
• W1978803247 doi "https://doi.org/10.1093/carcin/17.9.2003" @default.
• W1978803247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8824527" @default.
• W1978803247 hasPublicationYear "1996" @default.
• W1978803247 type Work @default.
• W1978803247 sameAs 1978803247 @default.
• W1978803247 citedByCount "37" @default.
• W1978803247 countsByYear W19788032472013 @default.
• W1978803247 countsByYear W19788032472014 @default.
• W1978803247 countsByYear W19788032472015 @default.
• W1978803247 countsByYear W19788032472017 @default.
• W1978803247 countsByYear W19788032472019 @default.
• W1978803247 countsByYear W19788032472021 @default.
• W1978803247 crossrefType "journal-article" @default.
• W1978803247 hasAuthorship W1978803247A5019253075 @default.
• W1978803247 hasAuthorship W1978803247A5062194105 @default.
• W1978803247 hasAuthorship W1978803247A5063367973 @default.
• W1978803247 hasAuthorship W1978803247A5067240879 @default.
• W1978803247 hasBestOaLocation W19788032471 @default.
• W1978803247 hasConcept C104317684 @default.
• W1978803247 hasConcept C109748351 @default.
• W1978803247 hasConcept C142724271 @default.
• W1978803247 hasConcept C153911025 @default.
• W1978803247 hasConcept C2776229224 @default.
• W1978803247 hasConcept C2778249689 @default.
• W1978803247 hasConcept C2779142324 @default.
• W1978803247 hasConcept C2779672484 @default.
• W1978803247 hasConcept C2908647359 @default.
• W1978803247 hasConcept C30481170 @default.
• W1978803247 hasConcept C502942594 @default.
• W1978803247 hasConcept C53226629 @default.
• W1978803247 hasConcept C54355233 @default.
• W1978803247 hasConcept C71924100 @default.
• W1978803247 hasConcept C86803240 @default.
• W1978803247 hasConcept C99454951 @default.
• W1978803247 hasConceptScore W1978803247C104317684 @default.
• W1978803247 hasConceptScore W1978803247C109748351 @default.
• W1978803247 hasConceptScore W1978803247C142724271 @default.
• W1978803247 hasConceptScore W1978803247C153911025 @default.
• W1978803247 hasConceptScore W1978803247C2776229224 @default.
• W1978803247 hasConceptScore W1978803247C2778249689 @default.
• W1978803247 hasConceptScore W1978803247C2779142324 @default.
• W1978803247 hasConceptScore W1978803247C2779672484 @default.

• next