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- W1978892743 abstract "We present a practical and efficient parentage analysis test for dogs. The method makes use of ten polymorphic microsatellite markers combined in three multiplex-PCR reactions. All three multiplexes can be performed under the same cycling conditions and using a multiple-dye detection system the PCR-products can be pooled for electrophoresis. Amplifications in four different thermal cyclers produced easily interpretable results throughout demonstrating the repeatability of the assay. In order to evaluate the method's efficiency a total of 100 dogs from four different breeds were typed. Assuming one known parent, exclusion probabilities ranging from 99.34% to 99.93% were attained." @default.
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- W1978892743 date "1999-04-01" @default.
- W1978892743 modified "2023-10-16" @default.
- W1978892743 title "A convenient and efficient microsatellite-based assay for resolving parentages in dogs" @default.
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- W1978892743 doi "https://doi.org/10.1046/j.1365-2052.1999.00438.x" @default.
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