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• W1978940325 abstract "The in vitro formation of organs and/or tissues is a major goal for regenerative medicine that would also provide a powerful tool for analyzing both the mechanisms of development and disease processes for each target organ. Here, we present a method whereby pancreatic tissues can be formed in vitro from mouse embryonic stem (ES) cells. Embryoid body-like spheres (EBSs) induced from ES cell colonies were treated with retinoic acid (RA) and activin, which are candidate regulators of pancreatic development in vivo. These induced tissues had decreased expression of the sonic hedgehog (shh) gene and expressed several pancreatic marker genes. ES cell-derived pancreatic tissue was composed of exocrine cells, endocrine cells, and pancreatic duct-like structures. In addition, the ratio of exocrine to endocrine cells in the induced tissue was found to be sensitive to the concentrations of RA and activin in the present experiment." @default.
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• W1978940325 date "2007-01-01" @default.
• W1978940325 modified "2023-09-30" @default.
• W1978940325 title "Pancreatic tissue formation from murine embryonic stem cells in vitro" @default.
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• W1978940325 doi "https://doi.org/10.1111/j.1432-0436.2006.00109.x" @default.
• W1978940325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17244017" @default.
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