FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978942017> ?p ?o ?g. }

• W1978942017 endingPage "671" @default.
• W1978942017 startingPage "664" @default.
• W1978942017 abstract "Despite much excellent work, one of the most fundamental problems in biology, namely, the mechanism through which the cell produces and reproduces sequentially specific heteropolymers (proteins, nucleic acids, polysaccharides, etc.), still remains far from being well understood. The nearest we have come to an understanding is through the postulation of template mechanisms in which a preformed polynucleotide prescribes, either directly by base pairing or through an intricate coding procedure, the order in which the precursors of the newly forming polymer, simultaneously or subsequently linked by enzyme action, are aligned. It is, perhaps, not surprising that the conventional concepts of enzymology are not entirely adapted to dealing with enzyme complexes whose action depends on, or is directed by, high-molecular templates that surpass the enzyme protein itself in size and often in complexity and sensitivity. We may describe the dilemma as follows. An enzyme can be defined by its substrate or by its product. If we define RNA polymerase, the enzyme studied here, by its substrate, the situation is clear: the ribonucleoside triphosphates are the only substrate and the enzyme is a ribonucleotidyl transferase. But in trying to define the enzyme by its product, we encounter a predicament of ever-shifting variables: there are as many products, and consequently as many enzymes, as there are templates. The central function of DNA in specifying the enzymic formation of polyribonucleotides of specific composition and, presumably, sequence, prompted us to investigate the problem of a template in some detail--a study that will be continued in subsequent communications. RNA polymerase (EC 2.7.7.6) has formed the subject of several recent reviews.1-3 The abolition or the alteration of template activity has been observed in several instances, e.g., on storage,4 irradiation with ultraviolet light5' 6 or X rays,7 and also by chemical modification.8' In the present study, we have used the enzyme preparation from E. coli and the DNA of calf thymus; we have investigated the changes in template activity exhibited by the latter when subjected to ultrasonic oscillations, changes in pH, and the partial removal of the purine constituents. The effects of temperature will be discussed later, as will be the actioln as templates of such extreme degradation products of DNA as apurinic and apyrimidinic acids. As we were naturally interested in both the quantity and the quality of the products of the enzyme action, we also examined the composition of the polyribonucleotides synthesized in the presence of the treated templates." @default.
• W1978942017 created "2016-06-24" @default.
• W1978942017 creator A5007567885 @default.
• W1978942017 creator A5019552217 @default.
• W1978942017 date "1966-08-01" @default.
• W1978942017 modified "2023-10-17" @default.
• W1978942017 title "Template functions in the enzymic formation of polyribonucleotides, I. Integrity of the DNA template." @default.
• W1978942017 cites W1507197532 @default.
• W1978942017 cites W1541782648 @default.
• W1978942017 cites W1555164544 @default.
• W1978942017 cites W1966026466 @default.
• W1978942017 cites W1986153343 @default.
• W1978942017 cites W1996490296 @default.
• W1978942017 cites W1999307070 @default.
• W1978942017 cites W2054046121 @default.
• W1978942017 cites W2067311371 @default.
• W1978942017 cites W2070124705 @default.
• W1978942017 cites W2078488174 @default.
• W1978942017 cites W2083197312 @default.
• W1978942017 cites W2409763212 @default.
• W1978942017 cites W2414420378 @default.
• W1978942017 cites W3119251453 @default.
• W1978942017 cites W59566502 @default.
• W1978942017 cites W75040750 @default.
• W1978942017 doi "https://doi.org/10.1073/pnas.56.2.664" @default.
• W1978942017 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/224424" @default.
• W1978942017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5338830" @default.
• W1978942017 hasPublicationYear "1966" @default.
• W1978942017 type Work @default.
• W1978942017 sameAs 1978942017 @default.
• W1978942017 citedByCount "17" @default.
• W1978942017 crossrefType "journal-article" @default.
• W1978942017 hasAuthorship W1978942017A5007567885 @default.
• W1978942017 hasAuthorship W1978942017A5019552217 @default.
• W1978942017 hasBestOaLocation W19789420171 @default.
• W1978942017 hasConcept C171250308 @default.
• W1978942017 hasConcept C185592680 @default.
• W1978942017 hasConcept C192562407 @default.
• W1978942017 hasConcept C552990157 @default.
• W1978942017 hasConcept C55493867 @default.
• W1978942017 hasConcept C70721500 @default.
• W1978942017 hasConcept C82714645 @default.
• W1978942017 hasConcept C86803240 @default.
• W1978942017 hasConceptScore W1978942017C171250308 @default.
• W1978942017 hasConceptScore W1978942017C185592680 @default.
• W1978942017 hasConceptScore W1978942017C192562407 @default.
• W1978942017 hasConceptScore W1978942017C552990157 @default.
• W1978942017 hasConceptScore W1978942017C55493867 @default.
• W1978942017 hasConceptScore W1978942017C70721500 @default.
• W1978942017 hasConceptScore W1978942017C82714645 @default.
• W1978942017 hasConceptScore W1978942017C86803240 @default.
• W1978942017 hasIssue "2" @default.
• W1978942017 hasLocation W19789420171 @default.
• W1978942017 hasLocation W19789420172 @default.
• W1978942017 hasLocation W19789420173 @default.
• W1978942017 hasLocation W19789420174 @default.
• W1978942017 hasOpenAccess W1978942017 @default.
• W1978942017 hasPrimaryLocation W19789420171 @default.
• W1978942017 hasRelatedWork W1967438259 @default.
• W1978942017 hasRelatedWork W2011396946 @default.
• W1978942017 hasRelatedWork W2028823067 @default.
• W1978942017 hasRelatedWork W2056619794 @default.
• W1978942017 hasRelatedWork W2059177774 @default.
• W1978942017 hasRelatedWork W2310302831 @default.
• W1978942017 hasRelatedWork W2411934966 @default.
• W1978942017 hasRelatedWork W2950945554 @default.
• W1978942017 hasRelatedWork W3034865888 @default.
• W1978942017 hasRelatedWork W3135111171 @default.
• W1978942017 hasVolume "56" @default.
• W1978942017 isParatext "false" @default.
• W1978942017 isRetracted "false" @default.
• W1978942017 magId "1978942017" @default.
• W1978942017 workType "article" @default.