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- W1978968548 abstract "Using preparation of rabbit ciliary body maintained in vitro, the electrical p.d. and the short circuit current were measured across the ciliary epithelium between the stromal and cameral sides. The bathing medium initially was a modified Krebs solution buffered with “Tris” and containing 5·5 mm glucose per 1. When citrate, isocitrate or succinate were added to or substituted for glucose in the bathing fluid the transepithelial potential increased by some 3·0 mV and the short circuit current by 45 to 50 μA.cm−2. With media containing sulphate in replacement for the chloride the increase of potential was exaggerated. The increased potential and short-circuit current is reversed upon addition of 0·03M malonate. Addition or substitution of oxaloacetate for glucose had no significant effect on potential or short circuit current. Over the ranges 7·06 < pH < 7·71 and 0·87 < [HCO3] < 32·1 there was no significant change of Na transport. On the basis that the short-circuit current gives a measure of active sodium transport from stroma to aqueous, it is suggested that this active process is driven from the citrate cycle. If the active transport of sodium is effected by a sodium-potassium-activated ATPase it seems likely that the production of ATP, required as a substrate, is coupled with aerobic oxidation of pyruvate via the citrate cycle and that glycolytic ATP is not available for this purpose. The failure of oxaloacetate to increase Na transport may be due to its utilization being limited by the turnover rate of the CoA-acetyl CoA system. It seems unlikely that CO2 uptake by the tissue plays an important role in its metabolism." @default.
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- W1978968548 date "1965-09-01" @default.
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- W1978968548 title "Citrate cycle and active sodium transport in rabbit ciliary epithelium" @default.
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- W1978968548 doi "https://doi.org/10.1016/s0014-4835(65)80034-3" @default.
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