Matches in SemOpenAlex for { <https://semopenalex.org/work/W1978973362> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1978973362 endingPage "64" @default.
- W1978973362 startingPage "57" @default.
- W1978973362 abstract "The biosynthesis of the carbohydrate antigen sialyl Lewis X (sLex) is dependent on the activity of an α3-fucosyltransferase (EC 2.4.1.152, GDP-fucose:Galβ(1–4)GlcNAc-R α(1–3)fucosyltransferase). This enzyme catalyses the transfer of fucose from GDP-β-fucose to the 3-OH of N-acetylglucosamine present in lactosamine acceptors. In this report, we have investigated the amino acids essential for the activity of a recombinant α3-fucosyltransferase (FucT-VI) through chemical modification of the enzyme with group-selective reagents. FucT-VI activity was found to be particularly sensitive to the histidine-selective reagent diethylpyrocarbonate and the cysteine reagent N-ethylmaleimide, with IC50 values of less than 200 μM. Reagents selective for arginine and lysine had no effect on enzyme activity. The inclusion of GDP-β-fucose during preincubation with NEM reduces the rate of inactivation whereas inclusion of an acceptor saccharide for the enzyme, Galβ(1–4)GlcNAc, had no effect. No protective effect with either GDP-β-fucose or Galβ(1–4)GlcNAc was observed on treatment of the enzyme with diethylpyrocarbonate. These data suggest that in addition to an NEM-reactive cysteine in, or adjacent to, the substrate-binding site of the enzyme, FucT-VI possesses histidine residue(s) that are essential for enzyme activity." @default.
- W1978973362 created "2016-06-24" @default.
- W1978973362 creator A5061584098 @default.
- W1978973362 creator A5066421259 @default.
- W1978973362 date "1997-02-01" @default.
- W1978973362 modified "2023-10-16" @default.
- W1978973362 title "Chemical modification of an α3-fucosyltransferase; definition of amino acid residues essential for enzyme activity" @default.
- W1978973362 cites W1967446962 @default.
- W1978973362 cites W1970757689 @default.
- W1978973362 cites W1971841464 @default.
- W1978973362 cites W1978364683 @default.
- W1978973362 cites W1984055823 @default.
- W1978973362 cites W1994711900 @default.
- W1978973362 cites W1997379663 @default.
- W1978973362 cites W2003157166 @default.
- W1978973362 cites W2015617142 @default.
- W1978973362 cites W2023689197 @default.
- W1978973362 cites W2031528659 @default.
- W1978973362 cites W2034731741 @default.
- W1978973362 cites W2040666939 @default.
- W1978973362 cites W2041310039 @default.
- W1978973362 cites W2071299534 @default.
- W1978973362 cites W2079657455 @default.
- W1978973362 cites W2083135897 @default.
- W1978973362 cites W2361497747 @default.
- W1978973362 cites W2418777549 @default.
- W1978973362 doi "https://doi.org/10.1016/s0304-4165(96)00076-1" @default.
- W1978973362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9042366" @default.
- W1978973362 hasPublicationYear "1997" @default.
- W1978973362 type Work @default.
- W1978973362 sameAs 1978973362 @default.
- W1978973362 citedByCount "27" @default.
- W1978973362 countsByYear W19789733622014 @default.
- W1978973362 countsByYear W19789733622020 @default.
- W1978973362 crossrefType "journal-article" @default.
- W1978973362 hasAuthorship W1978973362A5061584098 @default.
- W1978973362 hasAuthorship W1978973362A5066421259 @default.
- W1978973362 hasConcept C181199279 @default.
- W1978973362 hasConcept C185592680 @default.
- W1978973362 hasConcept C2776016237 @default.
- W1978973362 hasConcept C2776841590 @default.
- W1978973362 hasConcept C2777762822 @default.
- W1978973362 hasConcept C2778173381 @default.
- W1978973362 hasConcept C2778460671 @default.
- W1978973362 hasConcept C2779201268 @default.
- W1978973362 hasConcept C2781338088 @default.
- W1978973362 hasConcept C2781374117 @default.
- W1978973362 hasConcept C28212737 @default.
- W1978973362 hasConcept C515207424 @default.
- W1978973362 hasConcept C55493867 @default.
- W1978973362 hasConcept C71240020 @default.
- W1978973362 hasConceptScore W1978973362C181199279 @default.
- W1978973362 hasConceptScore W1978973362C185592680 @default.
- W1978973362 hasConceptScore W1978973362C2776016237 @default.
- W1978973362 hasConceptScore W1978973362C2776841590 @default.
- W1978973362 hasConceptScore W1978973362C2777762822 @default.
- W1978973362 hasConceptScore W1978973362C2778173381 @default.
- W1978973362 hasConceptScore W1978973362C2778460671 @default.
- W1978973362 hasConceptScore W1978973362C2779201268 @default.
- W1978973362 hasConceptScore W1978973362C2781338088 @default.
- W1978973362 hasConceptScore W1978973362C2781374117 @default.
- W1978973362 hasConceptScore W1978973362C28212737 @default.
- W1978973362 hasConceptScore W1978973362C515207424 @default.
- W1978973362 hasConceptScore W1978973362C55493867 @default.
- W1978973362 hasConceptScore W1978973362C71240020 @default.
- W1978973362 hasIssue "1" @default.
- W1978973362 hasLocation W19789733621 @default.
- W1978973362 hasLocation W19789733622 @default.
- W1978973362 hasOpenAccess W1978973362 @default.
- W1978973362 hasPrimaryLocation W19789733621 @default.
- W1978973362 hasRelatedWork W1976314208 @default.
- W1978973362 hasRelatedWork W1978973362 @default.
- W1978973362 hasRelatedWork W1985978334 @default.
- W1978973362 hasRelatedWork W1999674497 @default.
- W1978973362 hasRelatedWork W2004312381 @default.
- W1978973362 hasRelatedWork W2059277514 @default.
- W1978973362 hasRelatedWork W2064042198 @default.
- W1978973362 hasRelatedWork W2290318361 @default.
- W1978973362 hasRelatedWork W2386104817 @default.
- W1978973362 hasRelatedWork W2504449729 @default.
- W1978973362 hasVolume "1334" @default.
- W1978973362 isParatext "false" @default.
- W1978973362 isRetracted "false" @default.
- W1978973362 magId "1978973362" @default.
- W1978973362 workType "article" @default.