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- W1978976061 abstract "In the present paper the formation of complexes between positively charge polyelectrolyte (polyethyleneimine and chitosan) and Candida rugosa lipase from a crude extract and porcine lipase from pancreas commercial homogenate preparations were analyzed. The solubility of lipases-cationic polyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte and enzyme concentrations and salts present in the solution. The lipase was recovered from the non-soluble complex by adding of NaCl at a given pH. Although the polyelectrolytes did not affect lipase biological activity, both of them produced good enzyme recovery (>90%); however, purification factors were low. This methodology appears to be a good previous prepurification and concentration method, using, low-cost polymers, allows the design of a purification method where the protein of interest is present in a large volume with respect to the small amount of polyelectrolyte added." @default.
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- W1978976061 date "2011-10-01" @default.
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- W1978976061 title "Cationic polyelectrolytes–lipases complexes formation as tool for recovery of these enzymes from their natural sources" @default.
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- W1978976061 doi "https://doi.org/10.1016/j.ijbiomac.2011.05.012" @default.
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