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- W1978978510 abstract "We present here a new PCR-based technique that allows the production of several micrograms of DNA from only 300 flow-sorted chromosomes. During the first two PCR cycles, the annealing temperature is decreased to 30 °C, and numerous random loci are amplified under nonspecific conditions. As demonstrated here for pig chromosomes 1 and 18, the PCR products may be used to identify the chromosomal content of the flow-karyotype peaks of any species by fluorescence in situ hybridization." @default.
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- W1978978510 date "1993-01-01" @default.
- W1978978510 modified "2023-09-30" @default.
- W1978978510 title "A PCR-based method to amplify DNA with random primers: determining the chromosomal content of porcine flow-karyotype peaks by chromosome painting" @default.
- W1978978510 doi "https://doi.org/10.1159/000133457" @default.
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