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- W1979001230 abstract "Two human cDNA libraries prepared from normal fibroblast (GM3348) and rhabdomyosarcoma (CCL136) mRNAs were screened under cross hybridization conditions with a genomic fragment coding for exons 2 and 3 of the avian type III procollagen COOH-propeptide (Yamada, Y., Mudryj, M., Sullivan, M. and deCrombrugghe, B. (1983) J. Biol . Chem. 258, 2758–2761). One cDNA clone containing a 1.12 kb insert was isolated from the CCL136 library and later used to identify a GM3348 derived clone with a 2.4 kb insert. Comparison of the human and avian type III C-terminal propeptides revealed much more divergence in the first 54 amino acids following the terminal cysteine of the triple helical region than is present in the α1(I) and α2(I) procollagen chains of these species. Analysis of poly (A+) RNA from normal human fibroblast and tumor cell lines showed that they differed greatly in the relative amounts of α1(I), α2(I), and α1(III) mRNAs. Furthermore, as we previously reported for the α1(I) and α2(I) transcripts, multiple mRNAs also hybridize to the cloned α1(III) DNA." @default.
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- W1979001230 date "1984-01-01" @default.
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- W1979001230 title "Molecular cloning and carboxyl-propeptide analysis of human type III procollagen" @default.
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- W1979001230 doi "https://doi.org/10.1093/nar/12.24.9383" @default.
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