FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1979013707> ?p ?o ?g. }

• W1979013707 endingPage "6663" @default.
• W1979013707 startingPage "6652" @default.
• W1979013707 abstract "Several different approaches exist to generate expressed RNA interference (RNAi) precursors for multiple target inhibition, a strategy referred to as combinatorial (co)RNAi. One such approach makes use of RNA Pol III-expressed long hairpin RNAs (lhRNAs), which are processed by Dicer to generate multiple unique short interfering siRNA effectors. However, because of inefficient intracellular Dicer processing, lhRNA duplexes have been limited to generating two independent effective siRNA species. In this study, we describe a novel strategy whereby four separate anti-HIV siRNAs were generated from a single RNA Pol III-expressed transcript. Two optimized lhRNAs, each comprising two active anti-HIV siRNAs, were placed in tandem to form a double long hairpin (dlhRNA) expression cassette, which encodes four unique and effective siRNA sequences. Processing of the 3′ position lhRNA was more variable but effective multiple processing was possible by manipulating the order of the siRNA-encoding sequences. Importantly, unlike shRNAs, Pol III-expressed dlhRNAs did not compete with endogenous and exogenous microRNAs to disrupt the RNAi pathway. The versatility of expressed lhRNAs is greatly expanded and we provide a mechanism for generating transcripts with modular lhRNAs motifs that contribute to improved coRNAi." @default.
• W1979013707 created "2016-06-24" @default.
• W1979013707 creator A5017812913 @default.
• W1979013707 creator A5021667364 @default.
• W1979013707 creator A5053685911 @default.
• W1979013707 date "2010-06-04" @default.
• W1979013707 modified "2023-09-23" @default.
• W1979013707 title "Deriving four functional anti-HIV siRNAs from a single Pol III-generated transcript comprising two adjacent long hairpin RNA precursors" @default.
• W1979013707 cites W1488192195 @default.
• W1979013707 cites W1925504181 @default.
• W1979013707 cites W1970355156 @default.
• W1979013707 cites W1971643509 @default.
• W1979013707 cites W1972265894 @default.
• W1979013707 cites W1974343787 @default.
• W1979013707 cites W1974963758 @default.
• W1979013707 cites W1979390437 @default.
• W1979013707 cites W1979844428 @default.
• W1979013707 cites W1979925053 @default.
• W1979013707 cites W1982857973 @default.
• W1979013707 cites W1987583940 @default.
• W1979013707 cites W1990636994 @default.
• W1979013707 cites W1993883459 @default.
• W1979013707 cites W1994440449 @default.
• W1979013707 cites W1996804621 @default.
• W1979013707 cites W2000551195 @default.
• W1979013707 cites W2011927221 @default.
• W1979013707 cites W2015800866 @default.
• W1979013707 cites W2020787001 @default.
• W1979013707 cites W2030621748 @default.
• W1979013707 cites W2036108278 @default.
• W1979013707 cites W2036803556 @default.
• W1979013707 cites W2045663070 @default.
• W1979013707 cites W2049695132 @default.
• W1979013707 cites W2053632015 @default.
• W1979013707 cites W2056892724 @default.
• W1979013707 cites W2061113358 @default.
• W1979013707 cites W2063217898 @default.
• W1979013707 cites W2071088730 @default.
• W1979013707 cites W2072543946 @default.
• W1979013707 cites W2072901265 @default.
• W1979013707 cites W2075221603 @default.
• W1979013707 cites W2077169984 @default.
• W1979013707 cites W2078162078 @default.
• W1979013707 cites W2087453865 @default.
• W1979013707 cites W2088672050 @default.
• W1979013707 cites W2088835981 @default.
• W1979013707 cites W2089084676 @default.
• W1979013707 cites W2099414573 @default.
• W1979013707 cites W2102377924 @default.
• W1979013707 cites W2109590963 @default.
• W1979013707 cites W2115558774 @default.
• W1979013707 cites W2116660518 @default.
• W1979013707 cites W2125881252 @default.
• W1979013707 cites W2127509497 @default.
• W1979013707 cites W2131933548 @default.
• W1979013707 cites W2150505696 @default.
• W1979013707 cites W2151292912 @default.
• W1979013707 cites W2154681906 @default.
• W1979013707 cites W2171234781 @default.
• W1979013707 cites W2325262295 @default.
• W1979013707 doi "https://doi.org/10.1093/nar/gkq460" @default.
• W1979013707 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2965221" @default.
• W1979013707 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20525791" @default.
• W1979013707 hasPublicationYear "2010" @default.
• W1979013707 type Work @default.
• W1979013707 sameAs 1979013707 @default.
• W1979013707 citedByCount "36" @default.
• W1979013707 countsByYear W19790137072012 @default.
• W1979013707 countsByYear W19790137072013 @default.
• W1979013707 countsByYear W19790137072014 @default.
• W1979013707 countsByYear W19790137072015 @default.
• W1979013707 countsByYear W19790137072016 @default.
• W1979013707 countsByYear W19790137072017 @default.
• W1979013707 countsByYear W19790137072022 @default.
• W1979013707 crossrefType "journal-article" @default.
• W1979013707 hasAuthorship W1979013707A5017812913 @default.
• W1979013707 hasAuthorship W1979013707A5021667364 @default.
• W1979013707 hasAuthorship W1979013707A5053685911 @default.
• W1979013707 hasBestOaLocation W19790137071 @default.
• W1979013707 hasConcept C104317684 @default.
• W1979013707 hasConcept C145059251 @default.
• W1979013707 hasConcept C153911025 @default.
• W1979013707 hasConcept C166703698 @default.
• W1979013707 hasConcept C190232843 @default.
• W1979013707 hasConcept C2191507 @default.
• W1979013707 hasConcept C22615655 @default.
• W1979013707 hasConcept C2776802755 @default.
• W1979013707 hasConcept C2779867133 @default.
• W1979013707 hasConcept C54355233 @default.
• W1979013707 hasConcept C60723119 @default.
• W1979013707 hasConcept C67705224 @default.
• W1979013707 hasConcept C70721500 @default.
• W1979013707 hasConcept C86803240 @default.
• W1979013707 hasConcept C95444343 @default.
• W1979013707 hasConceptScore W1979013707C104317684 @default.
• W1979013707 hasConceptScore W1979013707C145059251 @default.
• W1979013707 hasConceptScore W1979013707C153911025 @default.
• W1979013707 hasConceptScore W1979013707C166703698 @default.

• next