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- W1979015110 abstract "Using immunohistochemistry we have been studying the postnatal maturation of vasoactive intestinal polypeptide (VIP)-positive neurons in organotypic slice cultures from rat visual cortex. The development in vitro is compared with the occurrence of VIP-containing cells in vivo, where they are first observed around postnatal day 5. A further increase in number and morphological maturation occurs within the following 3 weeks. In cultures prepared from 1- or 2-day-old rats, i.e. before VIP is expressed in vivo. VIP-containing neurons appear after about 5 days and gradually increase in number over the next 2 weeks. Thus the time course of postnatal expression of VIP in vitro and the morphology of VIP-immunoreactive neurons in culture closely matches the situation in vivo. These observations suggest that the maturation of VIP-containing neurons occurs independently of cortical afferents and that the intrinsic connectivity and activity is sufficient for their postnatal maturation. Therefore organotypic slice cultures should be a suitable system to study mechanisms of neurochemical maturation in the cortex." @default.
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- W1979015110 date "1989-12-01" @default.
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- W1979015110 title "Development of vasoactive intestinal polypeptide (VIP)-containing neurons in organotypic slice cultures from rat visual cortex" @default.
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- W1979015110 doi "https://doi.org/10.1016/0304-3940(89)90782-9" @default.
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