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• W1979017461 abstract "Nowadays, complaints about poor indoor air quality have become common. The variety of indoor air health problems include chronic fatigue, allergy, skin and eye irritation, and can be caused by several factors including fungi and their metabolites present in a building. The objective of this study was to establish a method for untargeted analysis of secondary fungal metabolites in indoor environments. As a detection technique, time-of-flight mass spectrometry was chosen, as it provided mass accuracy and higher sensitivity in full scan acquisition mode compared to tandem mass spectrometers. The method was first applied to fungal cultures, namely Penicillium brevicompactum and Chaetomium murorum, which were isolated from mouldy houses and grown on building materials under laboratory conditions for 7-21 days. Following the proposed strategy based on accurate mass measurement and post-acquisition data processing using principal component analysis, roquefortine C, brevianamide A and mycophenolic acid were identified in Penicillium sp., while chaetoglobosin A was found to be produced by Chaetomium sp. Subsequently, samples from mouldy inhabited buildings were analysed using the developed method. The actual presence of meleagrin was demonstrated in mouldy indoor environment. Applying the method to air and dust samples collected in these mouldy buildings, no metabolites were detected possibly due to generally low concentrations in these types of samples." @default.
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• W1979017461 date "2014-02-01" @default.
• W1979017461 modified "2023-10-16" @default.
• W1979017461 title "Untargeted screening of secondary metabolites in fungal cultures and samples from mouldy indoor environments by time-of-flight mass spectrometry" @default.
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• W1979017461 doi "https://doi.org/10.3920/wmj2013.1595" @default.
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