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• W197902825 abstract "The sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of X- and Y-chromosome-bearing sperm. This method is based on precise staining of the DNA of sperm with the nucleic acid-specific fluorophore, Hoechst 33342, to differentiate between the subpopulations of X- and Y-sperm. The fluorescently stained sperm are then sex-sorted using a specialized high speed sorter, MoFlo® SX XDP, and collected into biologically supportive media prior to reconcentration and cryopreservation in numbers adequate for use with artificial insemination for some species or for in vitro fertilization. Sperm sorting can provide subpopulations of X- or Y-bearing bovine sperm at rates in the 8,000 sperm/s range while maintaining; a purity of 90% such that it has been applied to cattle on a commercial basis. The sex of offspring has been predetermined in a wide variety of mammalian species including cattle, swine, horses, sheep, goats, dogs, cats, deer, elk, dolphins, water buffalo as well as in humans using flow cytometric sorting of X- and Y-sperm." @default.
• W197902825 created "2016-06-24" @default.
• W197902825 creator A5049729460 @default.
• W197902825 creator A5053755237 @default.
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• W197902825 date "2012-08-09" @default.
• W197902825 modified "2023-09-23" @default.
• W197902825 title "Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting" @default.
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• W197902825 doi "https://doi.org/10.1007/978-1-62703-038-0_26" @default.
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• W197902825 hasPublicationYear "2012" @default.
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