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• W1979036780 abstract "The plasma membrane potential of isolated rat hepatocytes was clamped at different values between 0 and -68 mV by addition of valinomycin in the presence of different extracellular concentrations of K+, and measured by the distribution of 86Rb+ between cells and medium. 36Cl- distribution came to steady state in 10-15 min. This steady-state distribution was compared to the plasma membrane potential over a range of values. 36Cl- distribution provided an accurate measurement of plasma membrane potential between -4 and -40 mV. At higher potentials intracellular chloride concentration is less than 20% of the extracellular concentration and errors due to uncertainties in the measurement of intracellular volume and of the contamination of cell pellets by extracellular medium precluded accurate determination of membrane potential: thus in our experiments 36Cl- underestimated the plasma membrane potential at -68 mV by 8 mV." @default.
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• W1979036780 date "1989-12-01" @default.
• W1979036780 modified "2023-09-27" @default.
• W1979036780 title "A quantitative assessment of the use of 36Cl− distribution to measure plasma membrane potential in isolated hepatocytes" @default.
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• W1979036780 doi "https://doi.org/10.1016/0005-2736(89)90462-8" @default.
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