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• W1979080960 abstract "INTRODUCTION: Melatonin (MEL), a pineal hormone, is well known as a potent antioxidant in a variety of ischemia-reperfusion models. Recent studies have assumed a pivotal role of reactive oxygen species (ROS) in the development of apoptosis. There are few pieces of information concerning a possible protective role of MEL against apoptosis in ischemia-reperfusion injury of myocardium. METHODS: We conducted an in vitro experiment: (1) to study the effect of MEL in the model of isolated and perfused working rat heart; (2) to evaluate the antioxidant capacity of MEL by a simple fluorescence test; and (3) to analyze the extent of apoptosis inhibition by MEL. Four groups of male Wistar rat were used: (a) group 'MEL 50 muM' (n=8); (b) group 'ischemia 30 min' (n=8); (c) group 'controls' (n=8); and (d) group 'controls+MEL 50 muM' (n=8). The perfusion medium was an oxygenated Krebs-Henseleit buffer (KHB). Hearts in groups (a) and (b) underwent 30 min of global normothermic ischemia and 45 min of reperfusion; 3 min before ischemia the hearts of group (a) received KHB with MEL 50 muM (and MEL 50 muM was also present in KHB solution during reperfusion). Hearts of group (c) were only perfused by KHB, and hearts of group (d) perfused by KHB+MEL 50 muM throughout the experiment. Registered were basic hemodynamic parameters: coronary, aortic, cardiac output and heart rate. At the end of each experiment, a left ventricle samples were taken for in situ detection of apoptosis using a TUNEL in-situ detection kit (POD) and quantitative analysis was performed. Malonedialdehyde concentrations were evaluated from heart homogenate to determine the severity of oxidative damage. To study the antioxidant capacity of MEL, a fluorescence test with allophycocyanin as an indicator was performed. A peroxyl radical generator, 2,2'-azobis(2-amidinopropan)-4-hydrochloride (AAPH) was used, and the antioxidant effect of MEL was expressed in oxygen-radical absorbing capacity (ORAC) units. RESULTS: Treatment by MEL resulted in a significant improvement of hemodynamic parameters and reduction of postischemic arrhythmias during reperfusion. All hearts in group 'ischemia 30 min' developed fatal ventricular fibrillations. MEL significantly reduced the incidence of apoptotic cells (14+/-4.3%; **P<0.01) vs. group 'ischemia 30 min' (58+/-2.1%). No apoptotic cells were detected in both control groups (c) and (d). In the fluorescence test, MEL exhibited a significant dose-dependent protective effect against peroxyl radical; MEL also reduced significantly the level of lipoperoxidation (MDA; *P<0.05). Analysis of hemodynamic parameters in both control groups (c) and (d) did not show any significant differences; the presence of MEL 50 muM in KHB solution did not have any important influence on cardiac performance in this type of experiment. CONCLUSION: We confirmed the previously reported beneficial effects of MEL against ischemia-reperfusion injury, presumably via its antioxidant properties. A significant suppression of apoptosis and the peroxyl radical scavenging properties of MEL in our study could contribute to the hypothesis of a close link between oxidative stress and apoptosis promotion." @default.
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• W1979080960 date "2003-05-01" @default.
• W1979080960 modified "2023-10-18" @default.
• W1979080960 title "Melatonin protects against ischemia-reperfusion injury and inhibits apoptosis in isolated working rat heart" @default.
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• W1979080960 cites W1972259716 @default.
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• W1979080960 cites W1983837006 @default.
• W1979080960 cites W198847946 @default.
• W1979080960 cites W1989713074 @default.
• W1979080960 cites W1990057346 @default.
• W1979080960 cites W1994290678 @default.
• W1979080960 cites W1995899406 @default.
• W1979080960 cites W2000665699 @default.
• W1979080960 cites W2002912123 @default.
• W1979080960 cites W2004851058 @default.
• W1979080960 cites W2015687370 @default.
• W1979080960 cites W2023025786 @default.
• W1979080960 cites W2024121244 @default.
• W1979080960 cites W2025943477 @default.
• W1979080960 cites W2037590153 @default.
• W1979080960 cites W2039292117 @default.
• W1979080960 cites W2049105093 @default.
• W1979080960 cites W2049326962 @default.
• W1979080960 cites W2053126056 @default.
• W1979080960 cites W2058232887 @default.
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• W1979080960 cites W2085651394 @default.
• W1979080960 cites W2088137315 @default.
• W1979080960 cites W2089199359 @default.
• W1979080960 cites W2092632324 @default.
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• W1979080960 cites W2144292389 @default.
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• W1979080960 doi "https://doi.org/10.1016/s0928-4680(02)00080-9" @default.
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